Smart cs program

The water maze test was performed in a laboratory with windows open for ventilation arranged with a table. A cylindrical water tank (1.5 m diameter * 12 cm height) was prepared with water at the temperature of 22°C-25°C and whole milk powder was dissolved to make a target not to be seen. The target (12 cm diameter) was place on the water tank floor about 1 cm below from the water surface. Escape distance and escape latency of an experimental animal was measured and analyzed after 12 weeks of experimental treatment utilizing SMART-CS program (Panlab, Barcelona, Spain), which is the computer program connected on the ceiling right above the water tank. The water maze test was conducted five days a week and mice were made to start from the same place and arrive at the target placed on the same location three times a day. On the last day of 6th, the mice were made to start swimming with the target removed, and two times of arrival at the target place were used as experimental value. Only when mice found the hidden target within 60 seconds, the obtained value was used as experimental result. Each examination was conducted with a minimum of five minute interval.