The hCMEC/D3 cells were treated with either vehicle (0.01% DMSO) or 17-DMAG (1 μM) for 24 h prior to the exposure to either vehicle (PBS) or H2O2 (10−4 M) for 8 h. Cells were then incubated with 2,7-Dichlorodihydrofluorescein diacetate (20 μM) for 30 min. The fluorescence intensity was measured in a Synergy HI Hybrid Multi-Mode Reader from Biotek (Winooski, VT).
Synergy hi hybrid multi mode reader
Manufactured by Agilent Technologies
The Synergy HI Hybrid Multi-Mode Reader is a versatile laboratory instrument that can perform a variety of detection methods, including absorbance, fluorescence, and luminescence. It is designed to provide accurate and reliable data for a wide range of applications, including cell-based assays, protein and nucleic acid quantification, and enzyme activity measurements.
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Lab products found in correlation
2 protocols using synergy hi hybrid multi mode reader
1
Oxidative Stress Evaluation in hCMEC/D3 Cells
2
Oxidative Stress Evaluation in hCMEC/D3 Cells
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The hCMEC/D3 cells were treated with either vehicle (0.01% DMSO) or 17-DMAG (1 μM) for 24 h prior to the exposure to either vehicle (PBS) or H2O2 (10−4 M) for 8 h. Cells were then incubated with 2,7-Dichlorodihydrofluorescein diacetate (20 μM) for 30 min. The fluorescence intensity was measured in a Synergy HI Hybrid Multi-Mode Reader from Biotek (Winooski, VT).
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