Drosophila embryo collection, fixation and antibody staining were carried out as previously described [40 (link)]. The following antibodies were used: FITC-conjugated goat anti-HRP (Jackson #123-095-021, 1:100), mouse anti-Fasciclin II (DSHB #1D4, 1:100), mouse anti-βgal (DSHB #40-1a, 1:150), mouse anti-Robo1 (DSHB #13C9, 1:100), rabbit anti-GFP (Invitrogen #A11122, 1:1000), mouse anti-HA (Covance #MMS-101P-500, 1:1000), Cy3-conjugated goat anti-mouse (Jackson #115-165-003, 1:1000), Alexa 488-conjugated goat anti-rabbit (Jackson #111-545-003, 1:500). Embryos were genotyped using balancer chromosomes carrying lacZ markers, or by the presence of epitope-tagged transgenes. Ventral nerve cords from embryos of the desired genotype and developmental stage were dissected and mounted in 70 % glycerol/PBS. Fluorescent confocal stacks were collected using a Leica SP5 confocal microscope and processed by Fiji/ImageJ [39 (link)] and Adobe Photoshop software.
Mms 101p 500
Manufactured by Jackson ImmunoResearch
The MMS-101P-500 is a laboratory equipment product manufactured by Jackson ImmunoResearch. It is a multi-channel magnetic separation stand designed for use with microplates or tubes. The product enables the separation of magnetic particles from liquid samples.
Automatically generated - may contain errors
Lab products found in correlation
Cy3 conjugated goat anti mouse, by Jackson ImmunoResearch (3 mentions)
Sp5 confocal microscope, by Adobe (3 mentions)
Fitc conjugated goat anti hrp, by Jackson ImmunoResearch (3 mentions)
Mouse anti ha, by Fortrea (3 mentions)
Alexa 488 conjugated goat anti rabbit, by Jackson ImmunoResearch (2 mentions)
Rabbit anti gfp, by Thermo Fisher Scientific (2 mentions)
Mouse anti fasciclin 2, by Developmental Studies Hybridoma Bank (2 mentions)
3 protocols using mms 101p 500
1
Drosophila Embryo Immunostaining Protocol
2
Drosophila Embryo Immunostaining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Drosophila embryo collection, fixation, and antibody staining were carried out as previously described (Hauptman et al., 2022 (link); Patel, 1994 (link)). The following antibodies were used: FITC-conjugated goat anti-HRP (Jackson ImmunoResearch #123–095-021, 1:100), mouse anti-Fasciclin II (Developmental Studies Hybridoma Bank [DSHB] #1D4, 1:100), mouse anti-βgal (DSHB #40–1a, 1:150), mouse anti-Robo3 cytoplasmic (DSHB #15H2, 1:100), mouse anti-HA (Covance #MMS-101P-500, 1:1000), Cy3-conjugated goat anti-mouse (Jackson #115–165-003, 1:1000). Embryos were genotyped using balancer chromosomes carrying lacZ markers. Ventral nerve cords from embryos of the desired genotype and developmental stage were dissected and mounted in 70% glycerol/PBS. Fluorescent confocal stacks were collected using a Leica SP5 confocal microscope and processed by Fiji/ImageJ (Schindelin et al., 2012 (link)) and Adobe Photoshop software.
3
Drosophila Embryo Immunostaining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Drosophila embryo collection, fixation and antibody staining were carried out as previously described (Patel 1994) . The following antibodies were used: FITC-conjugated goat anti-HRP (Jackson Immunoresearch #123-095-021, 1:100); mouse anti-Fasciclin II (Developmental Studies Hybridoma Bank [DSHB] #1D4, 1:100); mouse anti-βgal (DSHB #40-1a, 1:150); rabbit anti-GFP (Invitrogen #A11122, 1:1000); mouse anti-HA (Covance #MMS-101P-500, 1:1000); Cy3-conjugated goat anti-mouse (Jackson #115-165-003, 1:1000); Alexa 488-conjugated goat anti-rabbit (Jackson #111-545-003, 1:500). Embryos were genotyped using balancer chromosomes carrying lacZ markers, or by the presence of epitope-tagged transgenes. Ventral nerve cords from embryos of the desired genotype and developmental stage were dissected and mounted in 70% glycerol/PBS. Fluorescent confocal stacks were collected using a Leica SP5 confocal microscope and processed by Fiji/ImageJ (Schindelin et al. 2012) and Adobe Photoshop software.
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