Antibodies used were anti-TfR1 (no. 136800, Thermo Scientific, Waltham, MA, USA) and anti-tubulin (no. T5168, Sigma-Aldrich, Saint Louis, MO, USA). HRP-conjugated secondary antibodies used were anti-mouse (no. sc-516102) and anti-rabbit (no. sc-2357; Santa Cruz Biotechnology, Dallas, TX, USA). The chemicals used in this study were: Didox (3,4 dihydroxybenzohydroxamic acid; no. 10009081, Cayman Chemicals, MI, USA) and hydroxyurea (no. H8627, Sigma-Aldrich, Saint Louis, MO, USA) dissolved in dimethylsulfoxide (DMSO), three different well known iron chelators such as DFO (deferoxamine; no. S0080A, Novartis, Basel, Switzerland), DFP (deferiprone, kind gift of Prof. P. Ponka, University, Montreal, QC, Canada) and BPS (batophenantroline disulfonic acid, no. B1375); FAC (ferric ammonium citrate, no. F5879); FAS (ferrous ammonium sulfate, no. F2262); FeCl3 (ferric chloride, no. 157740) and ascorbic acid (no. A4034; Sigma-Aldrich, Saint Louis, MO, USA) all dissolved in water. Calcein (no. 21030 Sigma-Aldrich, Saint Louis, MO, USA), Calcein-AM (no. ALX-610-026 Calcein-acetoxymethyl ester, Enzo Life, Lausen, Switzerland) and MTT (thiazolyl blue tetrazolium bromide, no. M5655 Sigma-Aldrich, Saint Louis, MO, USA) were also used.
Anti tfr1
Manufactured by Thermo Fisher Scientific
Sourced in United States, France
The Anti-TfR1 is a lab equipment product that functions as a targeted antibody against the transferrin receptor 1 (TfR1) protein. TfR1 is a cell surface receptor involved in the cellular uptake of iron. The Anti-TfR1 is designed to specifically bind and detect the presence of TfR1 in various experimental and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti tubulin, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Hydroxyurea, by Merck Group (1 mentions)
Calcein, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Polyethylene glycol 6000, by Fujifilm (1 mentions)
Phorbol myristate acetate (pma), by Fujifilm (1 mentions)
Aurum total rna mini kit, by Bio-Rad (1 mentions)
Anti ftl, by Abcam (1 mentions)
Anti fth, by Santa Cruz Biotechnology (1 mentions)
Anti irp2, by Novus Biologicals (1 mentions)
Exosparkler exosome membrane labeling kit deep red, by Dojindo Laboratories (1 mentions)
Alexa 568 anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti cd63, by Thermo Fisher Scientific (1 mentions)
Anti α sma, by Abcam (1 mentions)
Autofluorescence quenching kit, by Vector Laboratories (1 mentions)
Erastin, by Selleck Chemicals (1 mentions)
Iron dextran, by Merck Group (1 mentions)
3 protocols using anti tfr1
1
Cellular Iron Homeostasis Regulation
2
Ferroptosis-related Exosome Analysis
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EMEM (Wako, 051–07615), FBS (Biowest, S1810-500; Nuaillé, France), exosome-depleted FBS (Exo-FBS™, Gibco, A27208-03), erastin (Selleck Chemicals, S7242), RSL3 (MedChemExpress, HY-100218A), ferric ammonium citrate (FAC; Sigma Aldrich, F5879), RhoNox-4 (Goryo Chemical; FerroOrange, GC904-01), ExoSparkler Exosome Membrane Labeling kit-Deep Red (Dojindo, EX03), phorbol 12-myristate 13-acetate (PMA; Wako, 162–23591), polyethylene glycol 6000 (Wako, 167–22941), Autofluorescence Quenching Kit (Vector, SP-8400), Aurum™ Total RNA Mini Kit (BioRad, 64337836), NEBNext Ultra II Directional RNA Library prep kit for Illumina (BioLabs, E7760S), NEBNext Multiplex Oligos for Illumina (BioLabs, E6440S), desferal (DFO, Novartis), anti-CD63 (Thermo Fischer Scientific, #10628D), anti-mesothelin (IBL, #28127), anti-αSMA (Abcam, ab5694), anti-CD68 (CST, 97778S), anti-GFP (MBL life science, #598), anti-TfR1 (Thermo Fischer Scientific, #13–6800), anti-FtL (abcam, ab69090), anti-FtH (SantaCruz, sc-H53), anti-IRP1 (CST, #20272), anti-IRP2 (Novus, NB100-1798), Cell Cycle Regulation Antibody Sampler Kit (CST, #9932), anti-mouse IgG Alexa488 (Thermo Fischer Scientific, A11108) and anti-rabbit IgG Alexa 568 (Thermo Fischer Scientific, A-11011) were used.
3
Quantifying Cellular Iron Homeostasis
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Antibodies used: anti-TfR1 (Cod. 136,800, Thermo Scientific, Waltham, MA), anti-Ferroportin (Cod. NBP1-21,502, Abnova, Novus Biologicals, Littleton, CO), and anti-Tubulin (Cod. T5168, Sigma-Aldrich, Saint Louis, MO). HRP-conjugated secondary antibodies used: anti-mouse (Cod: sc-516102, Santa Cruz Biotechnology, Dallas, TX) and anti-rabbit (Cod. A120-101P, Bethyl Laboratories, Inc., Montgomery, TX).
The chemicals dissolved in sterile deionized water: ferric ammonium citrate (FAC, Cod. F5879, Sigma-Aldrich, Saint Louis, MO), ascorbic acid (no. A4034, Sigma-Aldrich, Saint Louis, MO), Bathophenanthroline disulfonic acid disodium salt hydrate (BPS, Cod. B1375, Sigma-Aldrich, Saint Louis, MO), Desferoxamine (DFO, Cod. S0080A, Novartis), Deferiprone (DFP, kind gift of Prof. P. Ponka, University of Montreal, Canada); 3-[4, 5-dimethyl-2- thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT, Cod. M5655, Sigma-Aldrich, Saint Louis, MO).
Iron dextran (Cod. D851, Sigma-Aldrich, Saint Louis, MO) was diluted in saline buffer.
The chemicals dissolved in sterile deionized water: ferric ammonium citrate (FAC, Cod. F5879, Sigma-Aldrich, Saint Louis, MO), ascorbic acid (no. A4034, Sigma-Aldrich, Saint Louis, MO), Bathophenanthroline disulfonic acid disodium salt hydrate (BPS, Cod. B1375, Sigma-Aldrich, Saint Louis, MO), Desferoxamine (DFO, Cod. S0080A, Novartis), Deferiprone (DFP, kind gift of Prof. P. Ponka, University of Montreal, Canada); 3-[4, 5-dimethyl-2- thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT, Cod. M5655, Sigma-Aldrich, Saint Louis, MO).
Iron dextran (Cod. D851, Sigma-Aldrich, Saint Louis, MO) was diluted in saline buffer.
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