Genomic mini kit for dna isolation

Sodium valproate (Sanofi-Aventis, France) stock solution of 100 mg/ml in water was used to prepare the required concentration with a complete medium. Temozolomide (Merck, Germany) was dissolved in dimethyl sulfoxide (DMSO, Sigma) at a concentration of 0.103 M. [γ-P³²] ATP (6000 Ci/mmol) was purchased from Hartmann Analytic GmbH (Germany), T4 polynucleotide kinase was purchased from USB (UK), micrococcal nuclease, spleen phosphodiesterase II, apyrase, RNase P1, thiazolyl blue tetrazolium bromide, and inorganic salts were purchased from Sigma Aldrich/Merck (Germany), cellulose plates and methanol were purchased from Merck (Germany), and the Genomic Mini kit for DNA isolation was supplied by A&A Biotechnology (Poland).

Juglone and temozolomide (Merck, Darmstadt, Germany) were dissolved in dimethyl sulfoxide (DMSO, Sigma/Merck Darmstadt, Germany). [γ-³²P] ATP (6000 Ci/mmol) was purchased from Hartmann Analytic GmbH (Braunschweig, Germany). T4 polynucleotide kinase was purchased from USB Thermo Fisher Scientific, Waltham, MA, USA. Micrococcal nuclease, spleen phosphodiesterase II, apyrase, P1 nuclease, thiazolyl blue tetrazolium bromide, inorganic salts, cellulose plates, and methanol were purchased from Merck (Darmstadt, Germany). A Genomic Mini kit for DNA isolation was supplied by A&A Biotechnology (Gdańsk, Poland).