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Gipz lentiviral shrnamir vectors

Manufactured by Thermo Fisher Scientific
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Sourced in United States

GIPZ lentiviral shRNAmir vectors are a tool for RNA interference (RNAi) studies. They are designed to express small hairpin RNA (shRNA) molecules that can induce gene silencing in mammalian cells. The vectors contain the shRNAmir expression cassette, which includes the TurboGFP reporter and a puromycin resistance marker for selection.

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Lab products found in correlation

Rhs4346, by Thermo Fisher Scientific (1 mentions)

3 protocols using gipz lentiviral shrnamir vectors

1

Lentiviral shRNA Knockdown of Filaggrin

Cited 1 time
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Lentivirus production from GIPZ lentiviral shRNAmir vectors (Open Biosystems, Lafayette, Colo) targeting FLG (filaggrin knockdown short hairpin RNA [shFLG], V3LHS_369921) or a nontargeting control sequence (nontargeting control short hairpin RNA [shNT], RHS4346) and keratinocyte transduction were performed, as previously described.24 (link) Further details are provided in the Methods section in this article's Online Repository at www.jacionline.org.
Elias M.S., Long H.A., Newman C.F., Wilson P.A., West A., McGill P.J., Wu K.C., Donaldson M.J, & Reynolds N.J. (2017). Proteomic analysis of filaggrin deficiency identifies molecular signatures characteristic of atopic eczema. The Journal of Allergy and Clinical Immunology, 140(5), 1299-1309.
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2

Lentiviral shRNA Knockdown in HEK293T Cells

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HEK293T cells were cultured in complete DMEM containing 10% foetal calf serum. GIPZ lentiviral shRNAmir vectors were purchased from Open Biosystems (CO, USA) with distinct sequences targeting AhR (RHS4531, individual clones referred to as 1382 and 2320), empty GFP (RHS4349, EGFP) or non-silencing (RHS4346, NS) control sequences. 293T cells were co-transfected with pMD2.G envelope plasmid (5 μg), pCMVδ8.91 packaging plasmid (15 μg) and pGIPZ shRNA transfer plasmid (20 μg) using the calcium chloride precipitation method as previously described [17] , [18] (link).
Forrester A.R., Elias M.S., Woodward E.L., Graham M., Williams F.M, & Reynolds N.J. (2014). Induction of a chloracne phenotype in an epidermal equivalent model by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is dependent on aryl hydrocarbon receptor activation and is not reproduced by aryl hydrocarbon receptor knock down. Journal of Dermatological Science, 73(1), 10-22.
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3

Knockdown of Pitrilysin and IDE in INS 832/13 Cells

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GIPZ lentiviral shRNAmir vectors against rat pitrilysin (V3LMM_494519), rat IDE (V3LHS_312518) and a non-silencing control (RHS4346) were from Open Biosystems (Huntsville, AL, USA). These and other lentiviruses were produced according to the manufacturer’s instructions. For selection of INS 832/13 stable cells in which the level of pitrilysin or IDE was knocked-down, cells were transduced with the appropriate lentivirus and then selected with 10 μg/ml puromycin. Knockdown of pitrilysin or IDE was confirmed by Western blot analysis.
Guan H., Chow K.M., Song E., Verma N., Despa F, & Hersh L.B. (2015). The Mitochondrial Peptidase Pitrilysin Degrades Islet Amyloid Polypeptide in Beta-Cells. PLoS ONE, 10(7), e0133263.
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