Mouse anti human cd14 pacific blue

(Z‐Leu‐Leu‐NHCH2)2CO, 1,3‐di‐(N‐carboxybenzoyl‐ l‐leucyl‐ l‐leucyl)amino acetone, and 2,2′‐(2‐oxo‐1,3‐propanediyl)bis[N‐[(phenylmethoxy)carbonyl]‐ l‐leucyl‐ l‐leucinamide) ((Z‐LL₂)‐ketone) were purchased from Santa Cruz (sc‐311559); N‐morpholinurea–leucine–homophenylalanine–vinylsulfone‐phenol (LHVS) was synthesized in the laboratory of Dr. C Driessen, Department of Oncology and Hematology, Cantonal Hospital St Gallen, St Gallen, Switzerland, following protocols as published in [27]. Recombinant IFN‐γ was purchased from eBioscience (34‐8319‐82). The following antibodies were used: mouse anti‐human LAMP‐1 PE (BD; clone H4A3; 555801), mouse anti‐human CLIP FITC (BD; clone CerCLIP; 555981) mouse anti‐human CD74 PE (Biolegend; clone LN2; 326807), mouse anti‐human CD14 Pacific blue (Biolegend; clone M5E2; 301815), mouse anti‐human HLA ABC PE‐Cy7 (BD; clone G46‐2.6; 561349), mouse anti‐human HLA DR PerCP (Biolegend; clone L243; 560896), mouse anti‐human CD74 N‐terminal (Abcam; clone 2D1B3; ab181465), goat anti‐human β actin (Santa Cruz; clone I‐19; SC1616), goat anti‐mouse AF647 (Thermofisher Scientific; A28181), rabbit anti‐human Na‐K ATPase (Cell Signalling; 3010s), mouse anti‐human LAMP1 (BD, clone H4A3; 555798), Rabbit Anti‐mouse‐HRP antibody, (Dako; P0161), and Rabbit anti‐goat HRP antibody (Dako; P0160).

The following antibodies were used in flow cytometry assays: mouse anti-human CCR5, anti-human CXCR4 or anti-human CD4 (OKT4) (all PerCP-Cy5.5), mouse anti-human CD11c-PE-Cy7, mouse anti-human CD14-Pacific Blue and mouse anti-human CD16-APC-Cy7, all with corresponding isotype controls (Biolegend, San Diego, CA, USA). Fixable AquaDead or YellowDead Stain kits were from Molecular Probes (Invitrogen/Life Technologies, Waltham, MA, USA).