Microsoft excel 365

Statistical analyses and graphing were performed using Microsoft Excel 365 and GraphPad Prism 9.0 software (GraphPad, San Diego, CA, USA) or as an integral part of the computational pipelines. The Benjamini–Hochberg FDR method within AltAnalyze was used to calculate adjusted p values.

Heatmaps were generated with MetaboAnalyst 5.0 (www.metaboanalyst.ca). Statistical analysis was performed using Microsoft Excel 365™ and GraphPad Prism 8.4.3 software. For pairwise comparisons, two-tailed Student’s t-tests were used, and for multiple comparisons, one-way ANOVA test with Tukey’s post-hoc test. All error bars represent standard deviation (S.D.). All experimental data are representative of at least two independent experiments. Two biologically complementary in vivo metastasis experiments were performed. Images of mice for Fig. 2f were obtained from Servier Medical Art (https://smart.servier.com) under a Creative Commons Attribution 3.0 Unported License(https://creativecommons.org/licenses/by/3.0/). The mice in the images in Figs. 6a, 6h were obtained from Biorender (https://biorender.com/). The image in Fig. 4a was assembled in Powerpoint, Microsoft 365^®.

Statistical analyses for all data except those from lifespan assays were carried out using a t-test with appropriate parameters, i.e., a two-tailed unequal variances t-test used for comparison of the unpaired control vs. treatment groups. For comparing distributions between different groups in the lifespan assays, statistical calculations were performed using JMP software V9.0 (SAS Institute), applying the log-rank test. All other calculations were performed using Microsoft Excel 365 or GraphPad Prism V8.2.0 (GraphPad Software). P-values are reported in detail, without the use of arbitrary star ratings. However, in line with longstanding conventions used in the field, P-values <0.05 are generally referred to as indicating a significant difference, i.e., a rejection of the null hypothesis.