Indole 3 acetamide iam

Manufactured by Merck Group

Indole-3-acetamide (IAM) is a chemical compound used in laboratory settings. It is a derivative of indole and serves as a precursor in the biosynthesis of the plant growth hormone indole-3-acetic acid (IAA). IAM plays a role in various biochemical processes and is utilized in various research applications.

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Lab products found in correlation

P nf κb p65, by Cell Signaling Technology (1 mentions) Indole, by Merck Group (1 mentions) β actin c4 antibody, by Santa Cruz Biotechnology (1 mentions) Claudin 1 antibody, by Abcam (1 mentions) Lps derived from escherichia coli o111 b4, by Merck Group (1 mentions) L tryptophan trp, by Merck Group (1 mentions)

Close spelling variants of this product

Indole (92 citations) Acetamide (17 citations) Indole-3-acetamide (8 citations)

2 protocols using indole 3 acetamide iam

LPS-Induced Intestinal Barrier Disruption

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LPS derived from Escherichia coli O111:B4 was purchased from
Sigma-Aldrich (Burlington, MA, USA) and dissolved in phosphate-buffered saline
(PBS) to prepare the stock solutions with concentrations of 1 mg/mL.
DL-indole-3-lactic acid (ILA), 3-indoleacetic acid (IAA), indole-3-acetamide
(IAM), and indole were purchased from Sigma-Aldrich, and trifluoroacetic acid
was procured from Daejung Chemicals and Metals (Siheung, Korea). All reagents
were stored as specified by the manufacturer. The following antibodies were used
in the study: Zona occludens 1 (ZO-1) antibody (Cat No.21772-1-AP, Proteintech,
Chicago, IL, USA), claudin-1 antibody (Cat No. ab211737, Abcam, Cambridge, UK),
nuclear factor-kappa B (NF-κB) antibody (sc-372, Santa Cruz
Biotechnology, Dallas, TX, USA), p-NF-κB p65 (Cell Signaling Technology,
Danvers, MA, USA), β-actin C4 antibody (sc-4778, Santa Cruz
Biotechnology, Dallas, TX, USA), secondary R-antibody (Cat. No. A11036,
Invitrogen, Waltham, MA, USA), and Westar Supernova (Code.XLS3,0100, Cyanagen,
Srl, Bologna, Italy).

Wang X., Yong C.C, & Oh S. (2022). Metabolites of Latilactobacillus curvatus BYB3 and Indole Activate Aryl Hydrocarbon Receptor to Attenuate Lipopolysaccharide-Induced Intestinal Barrier Dysfunction. Food Science of Animal Resources, 42(6), 1046-1060.

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Tryptophan-Derived Metabolites in P. syringae

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P. syringae strains were grown in NYG medium with Rif in overnight cultures. Cells were collected by centrifugation from each overnight culture, washed twice with 10 mM MgCl₂, re-suspended at a density of ~1 x 10⁷ cells mL^-1 in HS minimal media containing 10 mM citrate and incubated with shaking for 48 hrs at 28°C. The culture medium was supplemented with 0.25 mM L-Tryptophan (Trp, Sigma Aldrich, Cat No. T-0254), Indole-3-acetamide (IAM, Sigma Aldrich, Cat No. 286281), 3-Indoleacetonitrile (IAN, Sigma Aldrich, Cat No. 129453), Tryptamine hydrochloride (TAM, Sigma Aldrich, Cat No 246557) or Indole-3-acetaldehyde–sodium bisulfite addition compound (IAAld, Sigma Aldrich, Cat No. I1000), as indicated. One mL samples were taken at 24 and 48 hrs after incubation, centrifuged to pellet the cells and the resulting supernatants frozen in liquid nitrogen and stored at -80°C. Growth of cultures was monitored by reading the OD₆₀₀ at regular intervals with a spectrophotometer. The samples were prepared and analyzed for IAA production by LC-MS/MS as described in Supplemental Information (S1 Text).

McClerklin S.A., Lee S.G., Harper C.P., Nwumeh R., Jez J.M, & Kunkel B.N. (2018). Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000. PLoS Pathogens, 14(1), e1006811.

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