Ccr4 pe cy7 1g1

Manufactured by BD

The CCR4-PE-Cy7 (1G1) is a fluorescently labeled monoclonal antibody that binds to the CCR4 receptor. It is designed for use in flow cytometry applications to identify and characterize cells expressing the CCR4 receptor.

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Lab products found in correlation

Cd4 alexafluor700, by BD (1 mentions) Lsr 2 cytometer, by BD (1 mentions) Cd4 fitc, by Beckman Coulter (1 mentions) Cd66b percp cy5.5 g10f5, by BioLegend (1 mentions) Cd19 percp cy5, by BioLegend (1 mentions) Ccr7 fitc 150503, by R&D Systems (1 mentions) Cd45ra apc efluor780 hi100, by Thermo Fisher Scientific (1 mentions) Hiv p24 rd1 fh190 1 1, by Beckman Coulter (1 mentions) Cd8 percp cy5, by BioLegend (1 mentions) Cd3 alexa700, by BioLegend (1 mentions) Hla dr bv785, by BioLegend (1 mentions) Cd3 pacific blue, by BD (1 mentions) Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Cd4 alexa700, by BD (1 mentions) Ki67 fitc, by BD (1 mentions) Cd8 fitc bw135 80, by Miltenyi Biotec (1 mentions) Cd19 fitc lt19, by Miltenyi Biotec (1 mentions) Ifn γ alexa fluor 700 b27, by BD (1 mentions) Cd45ra apc cy7, by BD (1 mentions)

Close spelling variants of this product

CCR4‐PE (3 citations)

2 protocols using ccr4 pe cy7 1g1

Polychromatic Flow Cytometry Analysis

Cited 1 time

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The following fluorochrome-conjugated Abs were used for polychromatic flow cytometry analysis: CD3-Pacific-blue (UCHT1), CD4-Alexa-Fluor 700 (RPA-T4), CCR4-PE-Cy7 (1G1), CCR6-PE (11A9), CCR7-PE-Cy7 (3D12), CXCR3-PE-Cy5 (1C6) (BD Biosciences, San Diego, CA), CCR7-FITC (150503) (R&D Systems, Minneapolis, MN), CD45RA-APC-eFluor780 (HI100) (eBioscience, San Diego, CA), CD3-Alexa700 (UCHT1), CD326-BV650 (9C4), CD8-PerCP-Cy5.5 (RPA-T8), CD19-PerCP-Cy5.5 (HIB19), CD66b-PerCP-Cy5.5 (G10F5), HLADR-BV785 (L243; BioLegend), CD4-FITC (SFCI12T4D11) and HIV-p24-RD1 (FH190-1-1; Beckman Coulter, Fullerton, CA). Live/Dead Fixable Aqua Dead Cell Stain Kit (Vivid; Life Technologies, Burlington, ON) was used to exclude dead cells. Cells were analyzed by FACS using the BD-LSRII cytometer and BD-Diva (BD Biosciences, San Jose, CA), and FlowJo (^©Tree Star, Inc., Ashland, OR) softwares. All Abs were titrated for an optimal noise/signal ratio and Ab cocktails were validated by comparing single to multiple staining. Positive gates were placed based on fluorescence minus one (FMO) controls [41 (link), 69 ]

Gosselin A., Wiche Salinas T.R., Planas D., Wacleche V.S., Zhang Y., Fromentin R., Chomont N., Cohen É.A., Shacklett B., Mehraj V., Ghali M.P., Routy J.P, & Ancuta P. (2016). HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy. AIDS (London, England), 31(1), 35-48.

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Multiparametric Flow Cytometry Analysis

Cited 1 time

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Fluorochrome-conjugated Abs used for polychromatic flow cytometry analysis were CD3-Pacific Blue (UCHT1), CD4-Alexa700 (RPA-T4), CD45RA-APC-Cy7 (custom), CCR4-PE-Cy7 (1G1), CXCR3-PE-Cy5 (1C6), CCR6-PE (11A9), Ki67-FITC, IFN-γ-AlexaFluor 700 (B27) (BD Pharmingen), CD56-FITC (MEM188), IL-17-PE (64DEC17) (eBioscience), HIV-p24-FITC (FH190-1-1) (Beckman Coulter), CD8-FITC (BW135/80), and CD19-FITC (LT19) (Miltenyi). A viability dye (Molecular Probes^® LIVE/DEAD^® Fixable Dead Cell Stain Kits, Invitrogen) was used to exclude dead cells. Cells were stained and analyzed by FACS using the BD LSRII cytometer and the FlowJo software, as previously described [129 (link)].

Cleret-Buhot A., Zhang Y., Planas D., Goulet J.P., Monteiro P., Gosselin A., Wacleche V.S., Tremblay C.L., Jenabian M.A., Routy J.P., El-Far M., Chomont N., Haddad E.K., Sekaly R.P, & Ancuta P. (2015). Identification of novel HIV-1 dependency factors in primary CCR4+CCR6+Th17 cells via a genome-wide transcriptional approach. Retrovirology, 12, 102.

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