Bicinchoninic acid bca protein assay kit

Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from HyClone (Logan, UT, USA). 100× Penicillin, streptomycin (P/S), and NO product kit were purchased from Sangon Biotech (Shanghai, China). Trypsin (0.25%) and Nuclear and Cytoplasmic Protein Extraction Kit were obtained from Beyotime (Shanghai, China). Dimethyl sulfoxide and MTT were purchased from Sigma–Aldrich (St. Louis, MO, USA). Enzyme-linked immunosorbent assay (ELISA) kits for mouse interleukin (IL)-1β and IL-10 were purchased from NeoBioscience (Shenzhen, China). LPS was sourced from Macklin (Shanghai, China). RNA extraction kit was purchased from Yuduo (Shanghai, China). SYBR Green polymerase chain reaction (PCR) kit and RevertAid First Strand cDNA Synthesis Kit were purchased from Thermo (Waltham, MA, USA). Bicinchoninic acid (BCA) Protein Assay Kit was obtained from Biosharp (Anhui, China). Polyvinylidene difluoride (PVDF) membranes were purchased from Millipore (Darmstadt, Germany). Phosphate-buffered saline (PBS) was sourced from Puhe (Wuxi, China). The primary antibodies (NF-Κb p65, TBP) used for western blot analyses were purchased from Abcam (Cambridge, London, UK); PKC-γ and GAPDH were sourced from HuaBio (Hangzhou, China) and Proteintech (Chicago, IL, USA), respectively. The JC was obtained from Nature's Sunshine Products Inc. (Shanghai, China).