Human il 17 elisa kit

All patients were treated initially with a topical application of 5% salicylic acid ointment for desquamation. Plaque scales were collected from the most representative lesions. The same amount of scale from each patient was weighed. Then, plaque scales were suspended in phosphate-buffered saline (PBS) without calcium and magnesium and disrupted with Sonics Vibracell VCX130 in impulsing mode. Cell suspension was centrifuged for 10 minutes at 5000 x g. Then, scale samples were subdivided into small aliquots to be stored at -80°C until tested for cytokine levels. The concentration of IL-17 in the patient plaque scales was determined with the use of Human IL-17A High Sensitivity ELISA kit (eBioscience, Vienna, Austria) and Human IL-17 ELISA kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturers' instructions. With Human IL-17A High Sensitivity ELISA kit, we have reached very high results, which were out of range. That is why we repeated the analysis with Human IL-17 ELISA kit (R&D Systems, Minneapolis, MN, USA).

Enzyme-linked immunosorbent assay (ELISA) was performed using Human IL-17 ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA) and Human IL-23 ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA) to measure the plasma IL-17 and IL-23 levels according to the manufacturer's instructions.