Human epithelial ovarian cancer cell lines, namely, OV-90 (MZ-2097, MINGZHOUBIO, Ningbo, China) and A2780 (CBP60283, Cobioer Biosciences, Nanjing, China), were stored in our laboratory. A2780—ovarian endometrioid adenocarcinoma. OV-90—High-grade serous ovarian cancer derived from metastatic site: ascites. Mouse epithelial ovarian cancer cell line ID-8 was stored in our laboratory. ID-8, OV-90, and A2780 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, C11995500BT) in an incubator with 5% CO2 at 37 °C. The complete medium contained 10% fetal bovine serum (FBS, Biological Industries, Israel, C04001500) and 1% penicillin–streptomycin (Cytiva, Logan, UT, USA, SV30010).
A2780
Manufactured by Cobioer Biosciences
Sourced in China
The A2780 is a cell line derived from human ovarian carcinoma. It is a widely used in vitro model for the study of ovarian cancer.
Automatically generated - may contain errors
Lab products found in correlation
Skov3, by Cobioer Biosciences (2 mentions)
Ov 90, by Thermo Fisher Scientific (1 mentions)
A2780, by Thermo Fisher Scientific (1 mentions)
C11995500bt, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Penicillin streptomycin, by Cytiva (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Caov 3, by Cobioer Biosciences (1 mentions)
Rpmi 1640, by Procell (1 mentions)
Mda mb 453, by Cobioer Biosciences (1 mentions)
Hcc38, by Cobioer Biosciences (1 mentions)
Hcc1806, by Cobioer Biosciences (1 mentions)
Hs 578t, by Cobioer Biosciences (1 mentions)
Ovcar 4, by Cobioer Biosciences (1 mentions)
Mda mb 231, by Cobioer Biosciences (1 mentions)
Skov3, by Thermo Fisher Scientific (1 mentions)
Lipofect 2000, by Thermo Fisher Scientific (1 mentions)
4 protocols using a2780
1
Ovarian Cancer Cell Line Cultivation
2
Ovarian Cancer Cell Transfection
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Normal ovarian epithelial cells IOSE80 and human ovarian cancer cell lines Caov-3, A2780, SKOV3, and CoC1 (COBIOER, Nanjing, China) were resuspended in calf serum-free RPMI1640 (PM150110, Procell Life Science & Technology, Wuhan, China). The cells were centrifuged at 1000 rpm for five min and incubated in cell medium with 15% fetal bovine serum (FBS) at 5% CO2 and room temperature. Then the Caov-3 cells and SKOV3 cells at logarithmic phase were seeded onto 6-well plates. When confluence reached 30%~60%, cells were transfected with short interfering RNA (si)-LINC00494, overexpression (oe)-LINC00494, oe-NFκB1, si-NFκB1, and oe-FBXO32 (Shanghai GenePharma Co, Ltd, Shanghai, China) using Lipofectamine 2000 reagents (#11669-027, Invitrogen, USA).
3
Ovarian and Breast Cancer Tissue and Cell Line Acquisition
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Archival FFPE tumor tissues were obtained from 195 ovarian and breast cancer patients who had signed the informed consents, and the study was approved by the Institutional Review Board of BGI Co., Ltd. 17 human cancer cells (HCC38, HCC1428, HCC1143, HCC1806, MX-1, HCC70, ZR-75-1, MDA-MB-453, MDA-MB-231, MDA-MB-361, MDA-MB-415, ZR-75-30, HS-578T, IGR-OV1, A2780, NCI/ADR-RES and OVCAR-4) and 3 matched-wildtype cell lines (HCC38BL, HCC1143BL and HCC1428BL) were purchased from the CoBioer Biosciences Co., Ltd. All cell lines have been verified by STR and were provided in the form of DNA status.
4
Overexpression of CAMK2D and SMARCA2
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A2780 and SKOV3 cells were purchased from Cobioer (Nanjing, China) and verified by Shanghai Biowing Applied Biotechnology Co., Ltd. Expression plasmids containing CAMK2D (NM_172115) and SMARCA2 (NM_003070) were purchased from Shanghai GeneChem Co., Ltd., (Shanghai, China) and Lipofect2000™ obtained from Invitrogen; Thermo Fisher Scientific, Inc.
Cisplatin-sensitive A2780 and SKOV3 cells were seeded into six-well plates and allowed to reach 90–95% confluence at 37°C before transfection. Cells were transfected with expression plasmids containing CAMK2D or SMARCA2 and those containing empty vector used as a control. The ratio of plasmid to Lipofect2000 was 1:2. After 6 h transfection, the medium was replaced with complete culture medium. Cells were continuously cultured until harvesting for analysis after 48 h transfection.
Cisplatin-sensitive A2780 and SKOV3 cells were seeded into six-well plates and allowed to reach 90–95% confluence at 37°C before transfection. Cells were transfected with expression plasmids containing CAMK2D or SMARCA2 and those containing empty vector used as a control. The ratio of plasmid to Lipofect2000 was 1:2. After 6 h transfection, the medium was replaced with complete culture medium. Cells were continuously cultured until harvesting for analysis after 48 h transfection.
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