Erythroid cells ter119

For negative enrichment of CD8⁺ T cells, the following biotinylated anti-mouse or anti-mouse/human antibodies were purchased from BioLegend: CD4 (clone RM4–5), CD19 (6D5), B220 (RA3–6B2), CD11b (M1/70), CD11c (N418), CD49b (DX5), and Erythroid cells (TER119). For negative selection of APCs, biotinylated anti-CD4 (RM4–5), CD8a (53–6.7), and Erythroid cells (TER119) were purchased from BioLegend. For flow cytometry, anti-CD126 (clone D7715A7), CD19 (6D5), CD25 (PC61), CD4 (RM4–5), CD44 (IM7), CD45.1 (A20), CD45.2 (104), CD62L (MEL-14), CD8 (53–6.7), Qa-2 (695H1-9-9), and TCR β chain (H57–597) were purchased from BioLegend. Anti-Bcl6 (clone K112–91), CD127 (SB/199), CD200 (OX-90), CD44 (IM7), CD5 (53-7.3), CD62L (MEL-14), CD71 (C2), CD8α (53–6.7), and Helios (22F6) were purchased from BD Biosciences. Anti-CD69 (clone H1.2F3) and FR4 (eBio12A5) were purchased from ThermoFisher Scientific. Streptavidin conjugated to APC (catalog #SA1005) and eFluor 450 (catalog #48-431782) were purchased from ThermoFisher Scientific. For viability, LIVE/DEAD fixable Near-IR, Violet or Yellow (ThermoFisher Scientific) was used according to the manufacturer’s instructions.