Neuronal nuclei (neun)

Manufactured by Wuhan Servicebio Technology

NeuN is a protein marker used to identify neuronal nuclei in tissue samples. It is a widely used tool in neuroscience research to visualize and quantify neurons.

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Lab products found in correlation

Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) Neuronal nuclei (neun), by Thermo Fisher Scientific (1 mentions) Anti connexin 43, by Wuhan Servicebio Technology (1 mentions) Wisteria floribunda lectin, by Vector Laboratories (1 mentions) Eclipse ti s, by Nikon (1 mentions)

3 protocols using neuronal nuclei (neun)

Immunohistochemical Analysis of Neuronal Death

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Brain tissues were fixed in paraformaldehyde (4%) and embedded in paraffin and sectioned in 5‐μm slices. The primary antibodies were anti‐glial fibrillary acidic protein (GFAP; Millipore), anti‐heme oxygenase‐1 (HO‐1; Servicebio), and anti‐connexin43 (Servicebio).
Neuronal cell death was measured using co‐staining of TUNEL and NeuN (a specific indicator for neurons). The fixed slices were stained using a Click‐iT TUNEL Imaging Assay kit (Thermo Fisher Scientific) and NeuN (Thermo Fisher Scientific), according to the instructions. The 4′,6‐diamidino‐2‐phenylindole (DAPI) was used as a nuclear specific marker.
The signals in the cortex in the peri‐ischemic region were observed (the focusing area is shown in Figure 1B) and images from three fields in the focusing areas were captured using a microscope (Nikon).

Qi Z., Yuan S., Liu K.J, & Ji X. (2022). Normobaric hyperoxia plays a neuroprotective role after cerebral ischemia by maintaining the redox homeostasis and the level of connexin43 in astrocytes. CNS Neuroscience & Therapeutics, 28(10), 1509-1518.

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Histochemistry and Immunohistochemistry Protocols

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Sections were processed for histochemistry, including Nissl staining and cytochrome oxidase staining (23 (link)), and immunohistochemistry as described previously (16 (link), 44 (link)–46 (link, link)). We visualized perineuronal nets using biotinylated Wisteria Floribunda Lectin (Vector; 1:1,000). First, we incubated sections in blocking buffer consisting of 0.1 M PBS and 5% BSA for 1 h at room temperature. Subsequently, free-floating sections were incubated at least overnight with the primary antibodies in 0.1 M PBS containing 1% BSA and 0.5% Triton-X (PBS-X). We used primary antibodies against PV (Swant; 1:5,000), Forkhead box protein P2 (Sigma; 1:500), NeuN (Servicebio; 1:1,000), and Purkinje cell protein 4 (Sigma; 1:500) (for details, see SI Appendix, Table S3). Next, sections were visualized with fluorescent secondary antibodies diluted in PBS-X and then mounted on gelatinized slides. All histological processing was performed with the experimenter being blind to whether the sections were from a shrew from the summer or winter group.

Ray S., Li M., Koch S.P., Mueller S., Boehm-Sturm P., Wang H., Brecht M, & Naumann R.K. (2020). Seasonal plasticity in the adult somatosensory cortex. Proceedings of the National Academy of Sciences of the United States of America, 117(50), 32136-32144.

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Immunofluorescence of Brain Tissue Sections

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For immunofluorescence of brain tissue sections, the brain was isolated and fixed by 4% paraformaldehyde in PBS overnight and then cut into 4 µm paraffin sections. After staining with primary antibody (ACSL4, Abcam, ab155282; NeuN, Servicebio, GB11138; EGFP, Servicebio, GB11602) and fluorescent-tagged secondary antibody, nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI), and coverslips were placed. Labeled sections were imaged with a confocal laser-scanning microscope (Nikon ECLIPSE Ti-S).

Tuo Q.Z., Liu Y., Xiang Z., Yan H.F., Zou T., Shu Y., Ding X.L., Zou J.J., Xu S., Tang F., Gong Y.Q., Li X.L., Guo Y.J., Zheng Z.Y., Deng A.P., Yang Z.Z., Li W.J., Zhang S.T., Ayton S., Bush A.I., Xu H., Dai L., Dong B, & Lei P. (2022). Thrombin induces ACSL4-dependent ferroptosis during cerebral ischemia/reperfusion. Signal Transduction and Targeted Therapy, 7, 59.

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