Gsh elisa kit

We used the SOD, MDA and GSH ELISA kit (Nanjing Jiancheng Bioengineering Institute, China) to detect the proteins from cell lysis. Quantitative assays were performed using the corresponding ELISA assay kit according to the manufacturer's protocol. The absorbance was measured at 550, 532 and 412 nm using a microplate reader (Thermo Fisher).

The iron deposition, iron content, and levels of MDA, GSH and GPX4 in mouse brains or cortical neurons were measured to evaluate the ferroptosis. The iron deposition in the brain was determined by Perls’ Prussian blue staining using the Perls’ stain Kit (Nanjing SenBeiJia Biological Technology, Nanjing, China) according to the manufacturer’s instructions. The iron content in mouse brains or cortical neurons was measured using the tissue iron assay kit (Nanjing Jiancheng, Nanjing, China) and the iron assay kit (Wuhan AmyJet Scientific, Wuhan, China), respectively. The contents of MDA, GSH, and GPX4 were measured using the MDA ELISA Kit (Nanjing Jiancheng, Nanjing, China), GSH ELISA Kit (Nanjing Jiancheng), and GPX4 ELISA Kit (Shanghai Enzyme Biotechnology, Shanghai, China), respectively.