A8994

Cells were cultivated at 37 °C in 21% O₂ and 5% CO₂ in a humidified incubator. The following melanoma cell lines and culture media were used: A375-P (R. Hynes, MIT), WM-266-4, A2058, SK-MEL-28, SK-MEL-2, MeWo and HEK-293T cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FCS (Ambimed), 2 mM L-glutamine (Gibco) and 1% non-essential amino acids (NEAA; Gibco). UACC-62 cells (NCI-60) were cultured in RPMI-1640 medium supplemented with 10% FCS and GlutaMax (Gibco). IGR-37 cells (ATCC) were cultured in DMEM supplemented with 15% FCS.
All cell lines were regularly tested for mycoplasma contamination with a PCR-based assay (A8994; AppliChem). Cell line profiling was performed using highly-polymorphic short tandem repeat loci (STRs) and the DNA profile was compared with databases (Microsynth).

The immortalized NP69 and NP460 nasopharyngeal epithelial cell lines were cultured in keratinocyte serum-free medium (Invitrogen, Carlsbad, CA, USA). The NPC cell lines, including CNE1, CNE2, C666-1, C666-1-shCPT1A, HK1, HONE1, HONE1-shCPT1A, HNE1, HNE2, HNE3, SUNE1, and SUNE1-CPTA were cultured in RPMI-1640 medium (Hyclone, Logan, UT, USA) supplemented with 10% FBS (Hyclone). All cells were cultured at 37 °C in a humidified incubator with 5% CO₂. Cells were cytogenetically tested and authenticated before being frozen. All cell lines were regularly tested for mycoplasma contamination by using a PCR-based assay (#A8994; AppliChem).