In Experiment I, the females were subjected to two different hormonal FTAI protocols (Figure 1 ). On day 0 (D0) at 8 h an intravaginal progesterone device (CIDR®) was introduced, then 2.0 mg of estradiol benzoate (Gonadiol®, Zoetis, Campinas, Brazil) was administered intramuscularly (IM). On day eight (D8) at 8 h, the progesterone implant was removed and administered by IM 2.5 mL of PGF2α (Lutalyse®) and 1 mg of estradiol cypionate (ECP®). At this time, heifers randomly received 300 IU of eCG (Novormon®) or 10 mg of FSH (Folltropin-V®) via IM. All hormones were from Zoetis (Campinas, Brazil) with exception of FSH which was bought from Vetoquinol Saúde Animal Ltd. (São Paulo, Brazil). On day ten (D10) from 8 am, the FTAIs were performed. In Experiment II, we evaluated the pregnancy rate of Nellore heifers subjected to FTAI with the same protocols containing FSH or eCG (Figure 1 ).
Gonadiol
Manufactured by Zoetis
Sourced in Brazil
Gonadiol is a laboratory equipment product manufactured by Zoetis. It is designed for the measurement and analysis of gonadal hormones. The core function of Gonadiol is to provide accurate and reliable data on the levels of hormones related to reproductive function.
Automatically generated - may contain errors
4 protocols using gonadiol
1
Hormonal FTAI Protocols in Nellore Heifers
2
Estradiol Cypionate for Estrus Synchronization in Beef Cows
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This study was performed at the experimental research farm of Embrapa Rondônia (08, 0 48′12″S, 63, 0 50′56″W).
Fourteen ovariectomized crossbred cows, 3-7 years old, 450-650 kg in body weight, and with a 3-4 of body condition score (BCS; range 1-5, where 1 = emaciated and 5 = obese; (Lowman et al., 1976 )) were used. Cows were kept in an outdoor grazing system (Brachiaria brizantha pasture) with ad libitum access to mineral, salt and water.
All cows were submitted to a TAI protocol and received an intravaginal progesterone-releasing device (1.9 g progesterone, CIDR®, Pfizer Animal Health, São Paulo, Brazil) plus 2 mg of estradiol benzoate (Gonadiol®, Zoetis, São Paulo, Brazil) i.m. on Day 0. On Day 7, cows received 12.5 mg i.m. of Dinoprost (PGF 2 α-analogue, Lutalyse®, Zoetis, São Paulo,Brazil). On Day 9, the CIDR devices were removed and cows were randomly assigned to two treatment groups to receive: 1) 0.5 mL of canola oil (Control Group, n = 14) i.m., or 2) 1 mg i.m. of estradiol cypionate (EC Group, n = 14; E.C.P®, Pfizer Animal Health, São Paulo, Brazil). A 2 × 2 cross-over design in which all cows received both treatments was used. The interval between replicates was 1 month.
Estrus detection aids (Estrotect®, Rockway Inc., Spring Valley, WI, USA) were placed on all cows on Day 9. Cows were observed for Estrotect® activation four times daily, from Days 9 to 13.
Fourteen ovariectomized crossbred cows, 3-7 years old, 450-650 kg in body weight, and with a 3-4 of body condition score (BCS; range 1-5, where 1 = emaciated and 5 = obese; (Lowman et al., 1976 )) were used. Cows were kept in an outdoor grazing system (Brachiaria brizantha pasture) with ad libitum access to mineral, salt and water.
All cows were submitted to a TAI protocol and received an intravaginal progesterone-releasing device (1.9 g progesterone, CIDR®, Pfizer Animal Health, São Paulo, Brazil) plus 2 mg of estradiol benzoate (Gonadiol®, Zoetis, São Paulo, Brazil) i.m. on Day 0. On Day 7, cows received 12.5 mg i.m. of Dinoprost (PGF 2 α-analogue, Lutalyse®, Zoetis, São Paulo,Brazil). On Day 9, the CIDR devices were removed and cows were randomly assigned to two treatment groups to receive: 1) 0.5 mL of canola oil (Control Group, n = 14) i.m., or 2) 1 mg i.m. of estradiol cypionate (EC Group, n = 14; E.C.P®, Pfizer Animal Health, São Paulo, Brazil). A 2 × 2 cross-over design in which all cows received both treatments was used. The interval between replicates was 1 month.
Estrus detection aids (Estrotect®, Rockway Inc., Spring Valley, WI, USA) were placed on all cows on Day 9. Cows were observed for Estrotect® activation four times daily, from Days 9 to 13.
3
Follicular Wave Synchronization in Cows
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cows satisfying the inclusion criteria received the following synchronization protocol to initiate a new follicular wave. On day 1, the cows received an intravaginal progesterone-releasing device (IPD; CIDR® [1.38 g of progesterone], Zoetis, Quito, Ecuador) + 2 mg of intramuscular estradiol benzoate (EB; Gonadiol®, Zoetis). Seven days later, in the morning, an intramuscular dose of 25 mg of prostaglandin (PG; Lutalyse® [dinoprost tromethamine], Zoetis) was applied and the implant was removed. On the following day (day 8), the cows received 1 mg of intramuscular EB (Gonadiol®) and, 54 h after removal of the implant, the location of the preovulatory follicle was explored by transrectal ultrasound (Aloka ProSound 2® , Tokyo, Japan) using a 7.5-MHz linear transducer. On day 11, which was considered the day of re-initiation of the follicular wave or 'day 0' of the study, the coccygeal vein was punctured, and blood was sampled into evacuated tubes containing ethylenediaminetetraacetic acid (BD Vacutainer®, Franklin Lakes, NJ, USA). Samples were immediately placed in a cooler with a cooling gel at 5 °C and centrifuged in the laboratory at 1500 g for 20 min. The plasma obtained was stored in aliquots at -20 °C until subsequent analysis of AMH concentrations. On day 11, AFC was also performed (the design is shown in online Supplementary Fig. S1).
4
Ovulation Synchronization for First TAI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following the expected voluntary waiting period of 55 ± 5 DIM, cows were enrolled into an ovulation synchronization protocol for first TAI and following negative pregnancy diagnoses.
Cows were presynchronized beginning at 32 ± 6 DIM using 2 injections of 25 mg (i.m.) of PGF 2α (dinoprost tromethamine; Lutalyse, Zoetis, SP, Brazil) given 14 d apart. Cows were then synchronized based on the protocol described by Pereira et al. (2015) (link): 11 d following the second PGF 2α injection of the presynchronization, cows initiated the following synchronization protocol: at d -11: intravaginal progesterone implant (Eazi-Breed controlled internal drug-releasing implant containing 1.9 mg of progesterone, Zoetis) + 2.0 mg (i.m.) of estradiol benzoate (Gonadiol, Zoetis) + 100 µg (i.m.) of gonadotropin-releasing hormone (gonadorelin diacetate; Cystorelin, Merial, SP, Brazil); at d -4: 25 mg (i.m.) of PGF 2α (dinoprost tromethamine; Lutalyse, Zoetis); at d -2: removal of intravaginal progesterone insert + 1.0 mg (i.m.) of estradiol cypionate (ECP, E.C.P., Zoetis) + 25 mg (i.m.) of PGF 2α ; and at d 0: TAI performed using commercial semen from 10 randomly selected bulls.
Positive pregnancy diagnosis was made when an amniotic vesicle with viable embryo was detected via rectal palpation with ultrasonography (Honda HS 101V with a 5.0-MHz linear probe, Honda, Tokyo, Japan) 32 and 60 d after TAI.
Cows were presynchronized beginning at 32 ± 6 DIM using 2 injections of 25 mg (i.m.) of PGF 2α (dinoprost tromethamine; Lutalyse, Zoetis, SP, Brazil) given 14 d apart. Cows were then synchronized based on the protocol described by Pereira et al. (2015) (link): 11 d following the second PGF 2α injection of the presynchronization, cows initiated the following synchronization protocol: at d -11: intravaginal progesterone implant (Eazi-Breed controlled internal drug-releasing implant containing 1.9 mg of progesterone, Zoetis) + 2.0 mg (i.m.) of estradiol benzoate (Gonadiol, Zoetis) + 100 µg (i.m.) of gonadotropin-releasing hormone (gonadorelin diacetate; Cystorelin, Merial, SP, Brazil); at d -4: 25 mg (i.m.) of PGF 2α (dinoprost tromethamine; Lutalyse, Zoetis); at d -2: removal of intravaginal progesterone insert + 1.0 mg (i.m.) of estradiol cypionate (ECP, E.C.P., Zoetis) + 25 mg (i.m.) of PGF 2α ; and at d 0: TAI performed using commercial semen from 10 randomly selected bulls.
Positive pregnancy diagnosis was made when an amniotic vesicle with viable embryo was detected via rectal palpation with ultrasonography (Honda HS 101V with a 5.0-MHz linear probe, Honda, Tokyo, Japan) 32 and 60 d after TAI.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!