Ab9485

After extraction of cell lysate and quantification of protein concentrations, 10% SDS-PAGE gels were used to separate proteins, and then they were transferred to 0.45 μm PVDF membranes (ThermoFisher, Waltham, MA, USA). After blocking the membranes with 5% non-fat milk, the membranes were incubated with primary antibodies of PLK1 (Abcam, Cambridge, UK, dilute 1 : 1000, ab189139) and GAPDH (Abcam, dilute 1 : 1000, ab9485) at 4 °C overnight, followed by incubation with a horseradish peroxidase-conjugated secondary antibody (Bioss, Beijing, China) at room temperature for 1 hour and visualization on a Tanon 5200 (Tanon, Shanghai, China).

After extraction of cell lysate and quantification of protein concentrations, proteins were separated on 10% SDS-PAGE gels and then transferred to 0.45 μm PVDF membranes (ThermoFisher, Waltham, MA, USA). The membranes were blocked with 5% nonfat milk, incubated with primary antibody of PXN (Abcam, Cambridge, UK, dilute 1 : 1000, ab32115) and GAPDH (Abcam, dilute 1 : 1000, ab9485) at 4°C overnight, treated with horseradish peroxidase-conjugated secondary antibody (Bioss, Beijing, China) at room temperature for 1 hour, and visualized on a Tanon 5200 (Tanon, Shanghai, China).