Steroidal and deuterated standards were purchased from Steraloids (Newport, RI, USA). The deuterated standard D7 cortisone [2,2,4,6,6,12,12-D7] and trimethylchlorosilane (TMCS) for the hydrolysis of steroids conjugates were from Sigma-Aldrich (St. Louis, MO, USA). Sylon BTZ, methoxyamine hydrochloride, and all other solvents and chemicals were from Merck (Darmstadt, Germany). All solvents were of HPLC grade.
Deuterated standard d7 cortisone 2 2 4 6 6 12 12 d7
Manufactured by Merck Group
Sourced in United States
Deuterated standard D7 cortisone [2,2,4,6,6,12,12-D7] is a stable isotope-labeled compound used as an internal standard in analytical methods for the quantification of cortisone in biological samples. It provides a well-defined and reproducible reference for accurate measurement of cortisone concentrations.
Automatically generated - may contain errors
3 protocols using deuterated standard d7 cortisone 2 2 4 6 6 12 12 d7
1
Steroidal Profiling with Deuterated Standards
2
Steroid Metabolomic Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Most steroids and deuterated standards used
for the metabolomic analysis were purchased from Steraloids (Newport,
RI, USA). The deuterated standard D7 cortisone [2,2,4,6,6,12,12-D7]
and trimethylchlorosilane (TMCS) for the hydrolysis of steroid conjugates
were from Sigma-Aldrich. Sylon BTZ, methoxyamine hydrochloride, and
all other solvents and chemicals were from Merck (Darmstadt, Germany).
All solvents were of high-performance liquid chromatography (HPLC)
grade.
Steroids used in the electrophysiological experiments
and to test the locomotor activity in the zebrafish: PA was prepared
as described,19 (link) 17-OH-PA was purchased
from Alfa chemistry (New York, USA, Catalogue No. A18D02141), and
PAG was prepared according to a published protocol.18 (link) Synthesis of 20-oxo-17α-hydroxy-5β-pregnan-3α-yll -glutamyl 1-ester and 17-OH-PAG is described below. Ketamine
hydrochloride and pentobarbital were purchased from Bioveta a.s. (Ivanovice
na Hane, Czech Republic).
for the metabolomic analysis were purchased from Steraloids (Newport,
RI, USA). The deuterated standard D7 cortisone [2,2,4,6,6,12,12-D7]
and trimethylchlorosilane (TMCS) for the hydrolysis of steroid conjugates
were from Sigma-Aldrich. Sylon BTZ, methoxyamine hydrochloride, and
all other solvents and chemicals were from Merck (Darmstadt, Germany).
All solvents were of high-performance liquid chromatography (HPLC)
grade.
Steroids used in the electrophysiological experiments
and to test the locomotor activity in the zebrafish: PA was prepared
as described,19 (link) 17-OH-PA was purchased
from Alfa chemistry (New York, USA, Catalogue No. A18D02141), and
PAG was prepared according to a published protocol.18 (link) Synthesis of 20-oxo-17α-hydroxy-5β-pregnan-3α-yl
hydrochloride and pentobarbital were purchased from Bioveta a.s. (Ivanovice
na Hane, Czech Republic).
3
Steroid Metabolite Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Most steroids and deuterated standards were purchased from Steraloids (Newport, RI, USA). The deuterated standard D7 cortisone [2,2,4,6,6,12,12-D7] and trimethylchlorosilane (TMCS) for hydrolysis of steroids conjugates were from Sigma-Aldrich (St. Louis, USA). Sylon BTZ, methoxyamine hydrochloride and all other solvents and chemicals were from Merck (Darmstadt, Germany). All solvents were of HPLC grade.
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