Gefitinib and erlotinib hydrochloride were obtained from LC Laboratories (Woburn, MA, USA) and Santa Cruz Biotechnology (Dallas, TX, USA), respectively. TUDCA and propidium iodide were commercially available from Sigma-Aldrich (St. Louis, MO, USA), while Hoechst 33342 and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were products of Dojindo Laboratories (Kumamoto, Japan). Tunicamycin and other chemical reagents were obtained from Wako Pure Chemicals (Osaka, Japan).
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt
Manufactured by Dojindo Laboratories
Sourced in Japan
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is a colorimetric assay used to measure the metabolic activity of cells. It is a water-soluble tetrazolium salt that is reduced to a formazan dye by the action of dehydrogenase enzymes in metabolically active cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Tunicamycin, by Fujifilm (1 mentions)
Tudca, by Merck Group (1 mentions)
Hoechst 33342, by Dojindo Laboratories (1 mentions)
Gefitinib, by LC Laboratories (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Erlotinib hydrochloride, by LC Laboratories (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
15d pgj2, by Cayman Chemical (1 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Bca protein assay reagent, by Thermo Fisher Scientific (1 mentions)
Deoxyribonuclease 1, by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Nacalai Tesque (1 mentions)
δ12 pgj2, by Cayman Chemical (1 mentions)
Biotinylated 15d pgj2, by Cayman Chemical (1 mentions)
Sequence grade modified trypsin, by Promega (1 mentions)
Anti phospho egfr tyr1068 antibody, by Cell Signaling Technology (1 mentions)
5 protocols using 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt
1
Evaluating Apoptotic Potential of Anti-Cancer Drugs
2
Proteomic Analysis of Lipid Mediators
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Leibovitz's/L15 medium, trypsin, deoxyribonuclease I, fetal bovine serum, horse serum, penicillin, and streptomycin were obtained from Invitrogen. PGD2, Δ12‐PGJ2, 15d‐PGJ2, and biotinylated 15d‐PGJ2 were obtained from Cayman Chemical. 3‐(4,5‐Dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H tetrazolium bromide (MTT) was purchased from DOJINDO. Immobiline™ DryStrip Gels (pH3‐10) and Amersham ECL Plus™ Western Blotting Detection Reagents were obtained from GE Healthcare Bio‐Sciences Corp. Sequence grade modified trypsin was purchased from Promega, and N‐(1‐pyrenyl) iodoacetamide was from Molecular Probes. Horseradish peroxidase‐linked antibody against biotin was obtained from Cell Signaling Technology. The protein concentration was measured using the BCA protein assay reagent obtained from Thermo Fisher Scientific. 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide (EDC) was obtained from Pierce. Hoechst 33342 was purchased from Nacalai Tesque. All other chemicals were of reagent grade (Yamamoto et al., 2015b ).
3
Quantification of Cellular Redox Signaling
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Epidermal growth factor (EGF), p-nitrophenyl phosphate (pNPP), and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 1,2-NQ (95% purity determined by HPLC), 1,4-NQ (100% purity determined by GC), and 1,4-BQ (99.1% purity determined by iodometric titration) were purchased from Tokyo Chemical Industry (Tokyo, Japan).
PD153035 and anti-PTP1B antibody (# PH01) were acquired from Calbiochem (San Diego, CA, USA). Biotin-(PEAC) 5 -maleimide (BPM) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from Dojindo Laboratories (Kumamoto, Japan). The anti-EGFR antibody (#2232), anti-phospho EGFR Tyr1068 antibody (#2234), anti-p44/42 MAP kinase antibody [anti-extracellular signal-regulated kinase 1/2 (ERK1/2), #9102], anti-phospho ERK1/2 antibody (#9101), horseradish peroxidase (HRP)-linked anti-rabbit IgG (#7074), and anti-mouse IgG (#7076) secondary antibody were procured from Cell Signaling Technology (Beverly, MA, USA). Fetal bovine serum, GlutaMAX-I, tris(2-carboxyethyl) phosphine hydrochloride (TCEP) and Ni-IDA ProBond were from Corning (Woodland, CA, USA), Gibco (Grand Island, NY, USA), Hampton Research (Aliso Viejo, CA, USA) and Invitrogen (Carlsbad, CA, USA), respectively. The anti-1,4-NQ antibody was prepared as previously reported (Hirose et al., 2012) . All other reagents used were of the highest purity available.
PD153035 and anti-PTP1B antibody (# PH01) were acquired from Calbiochem (San Diego, CA, USA). Biotin-(PEAC) 5 -maleimide (BPM) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from Dojindo Laboratories (Kumamoto, Japan). The anti-EGFR antibody (#2232), anti-phospho EGFR Tyr1068 antibody (#2234), anti-p44/42 MAP kinase antibody [anti-extracellular signal-regulated kinase 1/2 (ERK1/2), #9102], anti-phospho ERK1/2 antibody (#9101), horseradish peroxidase (HRP)-linked anti-rabbit IgG (#7074), and anti-mouse IgG (#7076) secondary antibody were procured from Cell Signaling Technology (Beverly, MA, USA). Fetal bovine serum, GlutaMAX-I, tris(2-carboxyethyl) phosphine hydrochloride (TCEP) and Ni-IDA ProBond were from Corning (Woodland, CA, USA), Gibco (Grand Island, NY, USA), Hampton Research (Aliso Viejo, CA, USA) and Invitrogen (Carlsbad, CA, USA), respectively. The anti-1,4-NQ antibody was prepared as previously reported (Hirose et al., 2012) . All other reagents used were of the highest purity available.
4
MTT Cell Viability Assay Protocol
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CA (Figure 1 ), Dulbecco’s Modified Eagle Medium (DMEM), Ham and Fetal bovine serum (FBS) were purchased from Sigma Aldrich Co., Ltd. (Irvine, United Kingdom). Penicillin and Streptomycin were purchased from Lonza Inc. (Walkersville, MD), while Eagle’s minimum essential medium (OPTI-MEM) was purchased from Gibco (Japan), and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) from DOJINDO, (Japan). SDS was purchased from GE Healthcare (Sweden).
5
Bovine Aortic Endothelial Cell Culture Protocol
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Bovine aortic endothelial cells were purchased from Cell Applications (San Diego, CA, USA). Tissue culture dishes and plates were from AGC Techno Glass (Shizuoka, Japan). Dulbecco's modified Eagle's medium (DMEM) and Ca 2+ -and Mg 2+ -free phosphate-buffered saline (CMF-PBS) were obtained from Nissui Pharmaceutical (Tokyo, Japan). Fetal bovine serum (FBS) and a bicinchoninic acid (BCA) protein assay kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2Htetrazolium bromide (MTT) was purchased from Dojindo Laboratories (Kumamoto, Japan). SbCl 3 and other reagents of the highest grade available were obtained from Nacalai Tesque (Kyoto, Japan).
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