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C 141

Manufactured by Alomone
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The C-141 is a laboratory instrument used for the detection and measurement of various analytes. It utilizes a specific detection method to provide quantitative data about the target compounds. The core function of the C-141 is to perform accurate and reliable analyses within a laboratory setting.

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Lab products found in correlation

Ab120003, by Abcam (3 mentions) T 550, by Alomone (2 mentions) Pm100a, by Thorlabs (2 mentions) M590l2 c2, by Thorlabs (2 mentions) S146c sensor, by Thorlabs (2 mentions) M470l2 c2, by Thorlabs (1 mentions)

3 protocols using c 141

1

Optogenetic Activation of Thalamocortical Fibers

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For activation of thalamocortical fibers, an extracellular stimulating electrode (concentric bipolar Pt/Ir electrode, 33 G; FHC) was placed in the external capsule in close proximity to visually identified mCherry-expressing fiber bundles. Fibers were activated by short, bipolar electrical pulses (400 μs, <100 μA). Opsins were activated using a 590 nm light emitting diode (LED; M590L2-C2; Thorlabs) delivered through the microscope illumination path which included a custom dichroic in order to reflect the 590 nm activation wavelength while collecting green fluorescence emission. Light power densities were calculated by measuring the light transmitted through the objective using a power meter (Thorlabs PM100A with S146C sensor) and dividing by the illumination area, calculated from the microscope objective field number and magnification25 (link). TTX (1 μM; T-550, Alomone), D-AP5 (25 μM; ab120003; Abcam) and CNQX (10 μM; C-141, Alomone) were bath applied where indicated.
Mahn M., Prigge M., Ron S., Levy R, & Yizhar O. (2016). Biophysical constraints of optogenetic inhibition at presynaptic terminals. Nature neuroscience, 19(4), 554-556.
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2

Precise Optical Stimulation in Neuronal Cultures

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Whole-field illumination in vitro was performed using a 470 nm light emitting diode (29 nm bandwidth LED; M470L2-C2; Thorlabs) delivered through the microscope illumination path including a custom dichroic in order to reflect the 470 nm activation wavelength. Light power densities were calculated by measuring the light transmitted through the objective using a power meter (Thorlabs PM100A with S146C sensor) and dividing by the illumination area, calculated from the microscope objective field number and magnification66 (link). D-AP5 (25 µM; ab120003; Abcam) and CNQX (10 µM; C-141, Alomone) were bath applied during all culture experiments. For spatially restricted illumination of neuronal soma or neurites, a 473 nm diode laser (Bruker) was directed to the imaging plane with galvanometric mirrors, yielding a diffraction-limited spot of light that provided brief light pulses (1 ms) at each location, with 500 ms inter-pulse intervals between non-adjacent locations.
Mahn M., Gibor L., Patil P., Cohen-Kashi Malina K., Oring S., Printz Y., Levy R., Lampl I, & Yizhar O. (2018). High-efficiency optogenetic silencing with soma-targeted anion-conducting channelrhodopsins. Nature Communications, 9, 4125.
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3

Optogenetic Activation of Thalamocortical Fibers

Check if the same lab product or an alternative is used in the 5 most similar protocols
For activation of thalamocortical fibers, an extracellular stimulating electrode (concentric bipolar Pt/Ir electrode, 33 G; FHC) was placed in the external capsule in close proximity to visually identified mCherry-expressing fiber bundles. Fibers were activated by short, bipolar electrical pulses (400 μs, <100 μA). Opsins were activated using a 590 nm light emitting diode (LED; M590L2-C2; Thorlabs) delivered through the microscope illumination path which included a custom dichroic in order to reflect the 590 nm activation wavelength while collecting green fluorescence emission. Light power densities were calculated by measuring the light transmitted through the objective using a power meter (Thorlabs PM100A with S146C sensor) and dividing by the illumination area, calculated from the microscope objective field number and magnification25 (link). TTX (1 μM; T-550, Alomone), D-AP5 (25 μM; ab120003; Abcam) and CNQX (10 μM; C-141, Alomone) were bath applied where indicated.
Mahn M., Prigge M., Ron S., Levy R, & Yizhar O. (2016). Biophysical constraints of optogenetic inhibition at presynaptic terminals. Nature neuroscience, 19(4), 554-556.
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