Anti tk

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-TK is a monoclonal antibody produced by Santa Cruz Biotechnology. It is designed to detect the Thymidine Kinase (TK) protein, which plays a role in DNA synthesis and cell proliferation. This antibody can be used in various research applications to study the expression and function of the TK protein.

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Lab products found in correlation

3 protocols using anti tk

Comprehensive Antibody Characterization Protocol

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The following antibodies were used: anti-SLFN5 (Sigma-Aldrich; Cat.HPA017760; Lot.B96361), anti-SLFN11 (Novus Biologicals; Cat.NBP1–92368; Lot.H96783), anti-PML (Bethyl Laboratory; Cat.A301–167A, Santa Cruz; Cat.sc-966; Lot.H1413), anti-IFI16 (Santa Cruz; Cat.sc-8023; Lot.C1312), anti-ATRX (Santa Cruz; Cat.sc-15408), anti-DNA-PKcs (Santa Cruz; Cat.sc-5282; Lot.G280), anti-SUMO2+3 (Abcam; Cat.ab3742; Lot.GR8249–1), anti-RNAP II (Santa Cruz; Cat.sc-56767), anti-GFP rabbit (Abcam; Cat.ab290; Lot.GR3251545–1), anti-GFP mouse (Millipore; Cat.MAB2510; Lot.2512480), anti-RAD50 (GeneTex; Cat.GTX70228; Lot.40186), anti-V5 (Santa Cruz; Cat.sc-271944; Lot.E2217), anti-HA (Abcam; Cat.ab9110; Lot.GR3217183–2), anti-GAPDH (GeneTex; Cat.GTX100118; Lot.42158), anti-α-Tubulin (Santa Cruz; Cat.sc-69969; Lot.DO412), anti-β-Actin (Sigma-Aldrich; Cat.a5441; Lot.064M4789V), anti-KU70 (Abcam; Cat.ab83501; Lot.GR3176811–2), anti-Histone H3 (Abcam; Cat.ab1791; Lot.GR3198176–1), anti-ICP0 (Santa Cruz; Cat.sc-53070; Lot. A0313), anti-ICP8 (gifted from David M. Knipe), anti-TK (Santa Cruz; Cat.sc-28037; Lot.K1813), anti-VP21 and anti-gD (gifted from Gary H. Cohen), anti-IE1/IE2 (Virusys; Cat.P1215; Lot.A1345070), anti-UL44 (Virusys; Cat.ca006–100; Lot.C1034151), adenovirus late protein antibody staining Hexon, Penton and Fiber (gift from James M. Wilson), and anti-DBP (gift from Arnold J. Levine).

Kim E.T., Dybas J.M., Kulej K., Reyes E.D., Price A.M., Akhtar L.N., Orr A., Garcia B.A., Boutell C, & Weitzman M.D. (2021). Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virus restriction factor that suppresses viral transcription. Nature microbiology, 6(2), 234-245.

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Quantifying Apoptosis Signaling Pathways

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The drugs, including Cln and GCV, were purchased from Sigma-Aldrich (St. Louis, MO, USA), and ICI 118551 hydrochloride (ICI) was purchased from Tocris Bioscience (Bristol, UK). The antibodies, including rabbit monoclonal anti-S100-β, rabbit polyclonal anti-Cx43, goat anti-rabbit IgG H&L (HRP), and goat polyclonal anti-rabbit IgG H&L (Alexa Fluor 488) were bought from Abcam (Cambridge, MA, USA). The rabbit polyclonal primary antibody against cleaved caspase-3 was purchased from Cell Signaling Technology (Beverly, MA, USA), anti-Bax, anti-Bcl-2, and anti-TK were bought from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The rabbit polyclonal anti-β-actin was obtained from PADZA (Tehran, Iran). DMEM/F12, fetal bovine serum (FBS), antibiotic/antimycotic, trypsin-EDTA, and MTT were purchased from Gibco Life Technologies (Carlsbad, CA, USA). The Annexin V/7-AAD detection kit, TRIzol reagent, and the reverse transcription kit were procured from BioLegend (San Diego, CA, USA), Thermo Fisher Scientific (Waltham, MA, USA), and Takara (Shiga, Japan), respectively. The enhanced chemiluminescence (ECL) kit was bought from Amersham Biosciences (Freiburg, Germany).

Hosseindoost S., Mousavi S.M., Dehpour A.R., Javadi S.A., Arjmand B., Fallah A, & Hadjighassem M. (2022). β2-Adrenergic receptor agonist enhances the bystander effect of HSV-TK/GCV gene therapy in glioblastoma multiforme via upregulation of connexin 43 expression. Molecular Therapy Oncolytics, 26, 76-87.

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Antibody Characterization for SLFN5, SLFN11, and DNA Repair

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The following antibodies were used: anti-SLFN5 (Sigma-Aldrich; Cat.HPA017760; Lot.B96361), anti-SLFN11 (Novus Biologicals; Cat.NBP1-92368; Lot.H96783), anti-PML (Bethyl Laboratory; Cat.A301-167A, Santa Cruz; Cat.sc-966; Lot.H1413), anti-IFI16 (Santa Cruz; Cat.sc-8023; Lot.C1312), anti-ATRX (Santa Cruz; Cat.sc-15408), anti-DNA-PKcs (Santa Cruz; Cat.sc-5282; Lot.G280), anti-SUMO2+3 (Abcam; Cat.ab3742; Lot.GR8249-1), anti-RNAP II (Santa Cruz; Cat.sc-56767), anti-GFP rabbit (Abcam; Cat.ab290; Lot.GR3251545-1), anti-GFP mouse (Millipore; Cat.MAB2510; Lot.2512480), anti-RAD50 (GeneTex; Cat.GTX70228; Lot.40186), anti-V5 (Santa Cruz; Cat.sc-271944; Lot.E2217), anti-HA (Abcam; Cat.ab9110; Lot.GR3217183-2), anti-GAPDH (GeneTex; Cat.GTX100118; Lot.42158), anti-α-Tubulin (Santa Cruz; Cat.sc-69969; Lot.DO412), anti-β-Actin (Sigma-Aldrich; Cat.a5441; Lot.064M4789V), anti-KU70 (Abcam; Cat.ab83501; Lot.GR3176811-2), anti-Histone H3 (Abcam; Cat.ab1791; Lot.GR3198176-1), anti-ICP0 (Santa Cruz; Cat.sc-53070; Lot. A0313), anti-ICP8 (gifted from David M. Knipe), anti-TK (Santa Cruz; Cat.sc-28037; Lot.K1813), anti-VP21 and anti-gD (gifted from Gary H. Cohen), anti-IE1/IE2 (Virusys; Cat.P1215; Lot.A1345070), anti-UL44 (Virusys; Cat.ca006-100; Lot.C1034151), adenovirus late protein antibody staining Hexon, Penton and Fiber (gift from James M. Wilson), and anti-DBP (gift from Arnold J. Levine).

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