Log In Sign up
The largest database of trusted experimental protocols

Bradford assay protocol

Manufactured by Bio-Rad
Visit Supplier
Sourced in United States

The Bradford assay protocol is a colorimetric method used to quantify the total protein concentration in a sample. It is based on the binding of the dye Coomassie Brilliant Blue G-250 to proteins, which results in a color change that can be measured spectrophotometrically. The protocol provides a simple and rapid way to determine protein levels in a wide range of sample types.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Bradford assay (3878 citations) Bradford protocol (2 citations)

2 protocols using bradford assay protocol

1

Bradford Assay for Protein Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein concentration was determined using the Bradford assay protocol (Bio-Rad Laboratories, Hercules, CA, USA), based on a standard curve generated with BSA.
Antos A., Topolska-Woś A., Woś M., Mitura A., Sarzyńska P., Lipiński T., Kurylcio A., Ziółkowski P., Świtalska M., Tkaczuk-Włach J., Gamian A., Polkowski W.P, & Staniszewska M. (2024). The unique monoclonal antibodies and immunochemical assay for comprehensive determination of the cell-bound and soluble HER2 in different biological samples. Scientific Reports, 14, 3978.
+ Open protocol
+ Expand
2

Tissue Protein Analysis in TMEV Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of tissue protein levels, TMEV infected or sham mice were perfused with ice cold PBS at 7 dpi before microdissection of the SVZ and cortex. SVZ cells were lysed on ice with lysis buffer (25 mM Tris-HCL pH 7.4, 150 mM NaCL, 1 mM EDTA, 1% NP-40 and 5% glycerol, 1 mM PMSF) for 15 min. Protein levels were measured using a standard Bradford assay protocol (Bio-Rad, Hemel Hempstead). For all protein experiments, 0.1 mg of tissue was used per sample and run in triplicate. For each experiment, tissue from 6 animals per group was pooled and replicated in three independent experiments. For analysis of supernatants, 100 μl of appropriate fluid dilutions were used (galectin-3 1:100). Cytokines were assayed from samples using respective ELISA kit according to the manufacturers instructions (R&D DuoSets).
James R.E., Hillis J., Adorján I., Gration B., Mundim M.V., Iqbal A.J., Majumdar M.M., Yates R.L., Richards M.M., Goings G.E., DeLuca G.C., Greaves D.R., Miller S.D, & Szele F.G. (2015). Loss of galectin-3 decreases the number of immune cells in the subventricular zone and restores proliferation in a viral model of multiple sclerosis. Glia, 64(1), 105-121.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!