Immunohistochemistry (IHC) assay was conducted as per the standard protocols. The monoclonal antibodies used for the analyses were as following: rabbit anti-CD66b (Abcam, Cambridge, UK), mouse anti-D2-40 (Dako, Carpinteria, USA), mouse anti-E-cadherin (E-cad) (Dako, Carpinteria, USA), rabbit anti-family with sequence similarity 3 member C (FAM3C) (Abcam, Cambridge, UK), rabbit anti-integrin α6 (Abcam, Cambridge, UK) and rabbit anti-CD151 (Affinity Biosciences, Cincinnati, USA) (for human tissue specimens), mouse anti-E-cad (Cell Signaling Technology, MA, USA), rabbit anti-vimentin (Vim) (Cell Signaling Technology, MA, USA) (for mouse allograft). Integrin α6 (ITGA6) and CD151 were detected using dual-color immunostaining in gastric tumor tissues. The expression intensity of E-cad or Vim was evaluated with mean density (MD) using Image-Pro Plus 6.0 image analysis software (Media Cybernetics, America), and MD means integral optical density (IOD) SUM divided by area SUM.
Mouse anti e cad
Manufactured by Cell Signaling Technology
Sourced in United States
Mouse anti-E-cad is a primary antibody that recognizes the E-cadherin protein in mouse samples. E-cadherin is a cell-cell adhesion molecule that plays a crucial role in maintaining the structural integrity of epithelial tissues.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti d2 40, by Agilent Technologies (1 mentions)
Image pro plus 6.0 image analysis software, by Media Cybernetics (1 mentions)
Ripa buffer, by Bio-Rad (1 mentions)
Bradford protein assay, by Bio-Rad (1 mentions)
Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions)
Mouse anti vimentin, by Cell Signaling Technology (1 mentions)
Anti tubulin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using mouse anti e cad
1
Immunohistochemistry Profiling of Gastric Tumor
2
Western Blot Analysis of Cellular Proteins
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Radioimmunoprecipitation assay (RIPA) buffer was used for the extraction of total cellular and tissue proteins (BioRad, Hercules, CA, USA). To determine the protein concentrations of the samples individually, a Bradford Protein Assay (BioRad) was carried out. Next, we normalized the total protein concentrations, and 50 µg of total protein was loaded into the wells of an 8–12% sodium dodecyl sulfate-polyacrylamide gel. We electrophoresized the proteins before transferring them to a polyvinylidene fluoride membrane (BioRad), which was subsequently incubated in the presence of primary mouse anti-human Dlg5 (1:1,000 dilution; Abcam, Cambridge, MA, USA), mouse anti-E-cad (1:500, Cell Signaling Technology), mouse anti-vimentin (1:1,000, Cell Signaling Technology), and anti-tubulin (1:3,000, Santa Cruz Biotechnology, CA, USA) for 16 h at 4 °C.
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