Aotosamdri 815

Manufactured by Tousimis

Sourced in Japan, United States

The Autosamdri-815 is a critical point dryer (CPD) designed for the drying of samples for scanning electron microscopy (SEM) analysis. The Autosamdri-815 uses liquid carbon dioxide to gently remove water from samples, preserving the original structure and morphology. This equipment is suitable for a variety of sample types, including biological and inorganic materials.

Automatically generated - may contain errors

Lab products found in correlation

Jec 3000fc, by JEOL (2 mentions) Su8010, by Hitachi (2 mentions) Supra 55, by Zeiss (1 mentions)

2 protocols using aotosamdri 815

Ultrastructure Analysis of Uterine Tissues

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At 4 °C, the natural uterus, decell uterus, and collagen were fixed for 4 h in 2.5% glutaraldehyde. Then, the samples were dehydrated for 15 min each in 30, 50, 75, 80, 90 vol% ethanol and 45 min in 100 vol% ethanol. After supercritical drying (aotosamdri‐815, Tousimis), the samples were sprayed with platinum (JEC‐3000FC, JEOL) for SEM observation (SU8010, Hitachi, Japan) and Field Emission Scanning Electron Microscopy (FE‐SEM) observation (SUPRA55, CARL ZEISS, Germany).

Gu Z., Guo J., Zhai J., Feng G., Wang X., Gao Z., Li K., Ji S., Wang L., Xu Y., Chen X., Wang Y., Guo S., Yang M., Li L., Han H., Jiang L., Wen Y., Wang L., Hao J., Li W., Wang S., Wang H, & Gu Q. (2022). A Uterus‐Inspired Niche Drives Blastocyst Development to the Early Organogenesis. Advanced Science, 9(28), 2202282.

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Preparing Samples for SEM Imaging

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For SEM, we began by fixing samples with 2.5% glutaraldehyde overnight at 4 °C. Samples were then dehydrated in 30%, 40%, 50%, 60%, 70%, 80%, and 90% ethanol for 15 min each, followed by dehydration in 100% ethanol three times. Dry samples were subjected to supercritical drying (aotosamdri-815, Tousimis, USA), then sprayed with platinum (JEC-3000FC, JEOL, Japan). Finally, samples were observed using SEM (SU8010, Hitachi, Japan).

Gao Z., Guo J., Gou B., Gu Z., Jia T., Ma S., Jiang L., Liu W., Zhou L, & Gu Q. (2023). Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness. Bioactive Materials, 28, 196-205.

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