Modular evo 800

Anthropometric measurements, including body mass index (BMI), height, weight, and waist circumference of participants, were performed at their residence by trained technicians using standardized procedures and portable equipment [26 (link)]. Blood pressure was measured twice using a sphygmomanometer according to standardized measurement techniques [26 (link)]; the mean of the readings was used in this study. Blood samples of total cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides, and glucose were collected from participants under an 8-h fasting period and measured using an enzymatic-colorimetric assay Modular Evo-800 (Roche Diagnostics, Mannheim, Germany). The Friedewald’s formula was used to calculate low-density lipoprotein (LDL) cholesterol [27 (link)]. Detailed explanations of the laboratory procedures are reported in the ENSANUT-ECU [23 ].

Participants’ height, weight, waist circumference, and blood pressure were measured twice at their residences by trained technicians using standardized procedures [34 (link)]. The mean of the readings was used for this study. Body mass index (BMI) was calculated from weight and height data (kg/m²). Fasting glucose, total cholesterol, HDL cholesterol, and triglyceride levels were measured from blood samples of participants who fasted for at least 8 h before examination. Blood samples were measured using an enzymatic-colorimetric assay Modular Evo-800 (Roche Diagnostics) and Friedewald’s formula was used to calculate LDL cholesterol [35 (link)]. The detailed laboratory procedures of ENSANUT-ECU are available elsewhere [23 ].
The subjects with MetS were identified when they had three or more of the following components: (1) waist circumference (men ≥ 94 cm, women ≥ 88), (2) blood pressure (systolic ≥ 130 mmHg and/or diastolic ≥ 85 mmHg), (3) HDL cholesterol (men < 40 mg/dL and women < 50 mg/dL), (4) elevated triglycerides (≥50 mg/dL), and (5) elevated fasting glucose (≥100 mg/dL). The diagnosis criteria were based on the National Cholesterol Education Program Adult Treatment Panel III and the Latin American Diabetes Association [36 (link),37 (link)].