The levels of the target proteins were measured with the following primary antibodies: anti-CNPase (1:1,000, ab6319, Abcam), anti-myelin basic protein (MBP; 1:1,000, ab62631, Abcam), anti-neuron glia 2 (NG2; 1:1,000, sc-33666, SANTA CRUZ), anti-GSK3β (1:1,000, D5C5Z, CST), anti-phosphorylated (Ser9) GSK3β (p-ser9-GSK3β; 1:1,000, D85E12, CST), anti-5-HT1AR (1:1,000, ab85615, Abcam), anti-human gene and protein symbol ACTB/ACTB (β-actin; 1:5,000, ab8226, Abcam), and anti-heat-shock protein 90 (HSP90; 1:2,000, ab203126, Abcam). The corresponding secondary antibodies (goat anti-rabbit IgG, CW0103 and goat anti-rabbit IgG, CW0102, CWBIO, P. R. China) for each protein were applied at a dilution of 1:1,000, followed by development with an ECL kit (AR1111, Boster, P. R. China). The band intensity was quantified using Image Lab software (version 5.2.1).
Sc 33666
Manufactured by Santa Cruz Biotechnology
Sc-33666 is a laboratory equipment product offered by Santa Cruz Biotechnology. The core function of this product is to provide a tool for scientific research and experimentation. No further details about the intended use or specifications of this product can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Ab62631, by Abcam (2 mentions)
Ab6319, by Abcam (2 mentions)
Ab85615, by Abcam (2 mentions)
Ab203126, by Abcam (2 mentions)
Ab8226, by Abcam (2 mentions)
Ecl kit, by Boster Bio (2 mentions)
Cw0102, by CWBIO (1 mentions)
Cw0103, by CWBIO (1 mentions)
Ab155279, by Abcam (1 mentions)
Ab89780, by Abcam (1 mentions)
Ab23631, by Abcam (1 mentions)
Ab8245, by Abcam (1 mentions)
2 protocols using sc 33666
1
Quantification of Target Proteins by Immunoblotting
2
Comprehensive Protein Analysis in Neuroscience
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The target proteins were measured with the following primary antibodies: anti-2'3'-cyclic nucleotide 3'phosphodiesterase (CNPase; mouse, 1:1000, ab6319, Abcam), anti-myelin basic protein (MBP; mouse, 1:1000, ab62631, Abcam), anti-NG2 (NG2; mouse, 1:1000, sc-33666, SANTA CRUZ), anti-SOX10 (SOX10; rabbit, 1:1000, ab155279, Abcam), anti-GSK3β (rabbit, 1:1000, D5C5Z, CST), anti-phosphorylated (Ser9) GSK3β (p-ser9-GSK3β; rabbit, 1:1000, D85E12, CST), anti-5-HT1AR (rabbit, 1:1000, ab85615, Abcam), antileucine-rich repeat and Ig domain containing 1 gene (LINGO1; rabbit, 1:1000, ab23631, Abcam), antimyelin-associated glycoprotein (MAG; mouse, 1:1000, ab89780, Abcam), anti-myelin oligodendrocyte glycoprotein (MOG; rabbit, 1:1000, ab233549, Abcam), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; rabbit, 1:5000, ab8245, Abcam), anti-human gene and protein symbol ACTB/ACTB (β-actin; mouse, 1:5000, ab8226, Abcam) and anti-heat-shock protein 90 (HSP90; rabbit, 1:2000, ab203126, Abcam). The corresponding secondary antibodies for each protein were used at a dilution of 1:1000, followed by development with an ECL kit (AR1111, Boster, China).
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