On Day 14 of osteogenic induction, MSCs were fixed with 4% paraformaldehyde for 30 min and then stained with 1% ARS (pH 4.3) for 15 min. After washing three times with deionized water, the calcium deposits were observed and imaged with a Nikon Eclipse Ti-S inverted microscope. To quantify calcium deposits, the stained cells were destained with 10% cetylpyridinium chloride monohydrate (CPC, Sigma–Aldrich, USA) in 10 mM sodium phosphate (pH = 7.0) for 1 h. A 200 μl aliquot was transferred to a 96-well plate, and the absorbance was measured at 562 nm by a Varioskan Flash Spectral Scanning Multimode Reader (Thermo Fisher Scientific Inc, USA).
Cetylpyridinium chloride monohydrate cpc
Manufactured by Merck Group
Sourced in United States
Cetylpyridinium chloride monohydrate (CPC) is a chemical compound that functions as a surfactant and disinfectant. It is a white crystalline powder.
Automatically generated - may contain errors
2 protocols using cetylpyridinium chloride monohydrate cpc
1
Quantification of Osteogenic Mineralization
2
Aluminum Alloy Surface Modification
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Aluminum alloy (SAE AMS 4037) was provided by Kaiser Aluminum Corporation and cut into 50 mm × 25 mm × 2 mm pieces. Hydrochloric acid (37%, w/w), hydrogen peroxide (30%, w/w), petroleum ether (60–90°C), dichloromethane, ethanol, and acetone were supplied by Chengdu Kelong Chemical Company. Cetylpyridinium chloride monohydrate (CPC) was obtained from Sigma-Aldrich. NaOH was obtained from Guangdong Chemical Reagent Engineering-Technological Research and Development Center.
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