Anti cd8 percp cy5.5 sk1

Manufactured by BD

Anti-CD8-Percp-Cy5.5 (SK1) is a monoclonal antibody conjugated with PerCP-Cy5.5 dye. It is designed to detect and analyze CD8-positive cells in flow cytometry applications.

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Lab products found in correlation

Anti ccr7 bv785, by BioLegend (1 mentions) Anti cd3 bv510 hit3a, by BD (1 mentions) Nlvpmvatv, by JPT Peptide Technologies (1 mentions) Anti cd45ra apc cy7 hi100, by BioLegend (1 mentions) Facs lysing solution, by BD (1 mentions)

Spelling variants of this product

Anti-CD8 (193 citations) PerCP-Cy5.5 (120 citations) CD8-PerCP (53 citations) CD8-PerCP-Cy5.5 (47 citations) Anti-CD8-PerCP (46 citations) Anti-CD8-PerCP-Cy5.5 (45 citations) CD8 (SK1, (19 citations) PerCP-Cy5.5-CD8 (11 citations) Anti-CD8 (SK1, (8 citations) PerCP/Cy5.5-CD8 (SK1; (2 citations)

2 protocols using anti cd8 percp cy5.5 sk1

Phenotyping CMV-specific T Cells

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Cryopreserved PBMCs were stained with cell trace violet (CTV; Thermo Fisher) (20 minutes, RT), and then stimulated with 1 μg/mL epitope peptide (NLVPMVATV; JPT Peptide Technologies) for 6 days at 37°C in 500 μL of 10% fetal bovine serum/RPMI 1640 medium (WELGENE). Cells were labeled with HLA-A∗0201-restricted CMVpp65 pentamer (HLA-A2∗0201: CMVpp56_495, ProImmune). Next, surface molecule staining, dead cell staining, fixation, permeabilization, and intracellular molecule staining were performed using the following antibodies: anti-CD3-BV510 (HIT3a), anti-CD8-Percp-Cy5.5 (SK1; BD Biosciences), anti-CD19-PE-eFluor610 (HIB19), anti-CD14-PE-eFluor610 (61D3), anti-Ki-67-PE-Cy7 (20Raj1; Thermo Fisher), anti-CD45RA-APC-Cy7 (HI100), and anti-CCR7-BV785 (G043H7; BioLegend), and a Foxp3 Staining Buffer Set. Data were collected using an LSR II instrument.

Kim S., Lee S.W., Koh J.Y., Choi D., Heo M., Chung J.Y., Lee B.H., Yang S.H., Sung Y.C., Lee H., Shin E.C, & Park S.H. (2022). A single administration of hIL-7-hyFc induces long-lasting T-cell expansion with maintained effector functions. Blood Advances, 6(23), 6093-6107.

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PBMC Proliferation Assay for Mtb Antigen

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A six-day proliferation assay was performed as previously described [30] (link). Briefly, freshly isolated peripheral blood mononuclear cells (PBMCs) were labeled with CellTrace Oregon Green 488 carboxylic acid diacetate, succinimidyl ester (OG; Life Technologies). OG-labeled cells were incubated for 6 days with either Mtb peptide pools, or the positive control SEB, or no antigen (negative control). Cells were then stained with LIVE/DEAD Fixable Violet Dead Cell Stain (Vivid; Life Technologies), fixed with FACS Lysing Solution (BD Biosciences), and stained with anti-CD4 Qdot605 (S3.5, Life Technologies), anti-CD3 allophycocyanin-H7 (SK7), and anti-CD8 PerCP-Cy5.5 (SK-1), both from BD Biosciences.

Day C.L., Moshi N.D., Abrahams D.A., van Rooyen M., O'rie T., de Kock M, & Hanekom W.A. (2014). Patients with Tuberculosis Disease Have Mycobacterium tuberculosis-Specific CD8 T Cells with a Pro-Apoptotic Phenotype and Impaired Proliferative Capacity, Which Is Not Restored following Treatment. PLoS ONE, 9(4), e94949.

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