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Rat aortic endothelial cells

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Rat aortic endothelial cells are primary cells isolated from the aorta of rats. They are useful for in vitro studies of endothelial cell biology and function.

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Lab products found in correlation

Heparin, by Sandoz (1 mentions) α tubulin, by Merck Group (1 mentions) Vinculin, by Merck Group (1 mentions) Atp determination kit, by Thermo Fisher Scientific (1 mentions) G actin f actin in vivo assay biochem kit, by Cytoskeleton (1 mentions) P38 mapk, by Merck Group (1 mentions) 3h triolein, by PerkinElmer (1 mentions) Hp3 17, by ProSpec (1 mentions) P p38mapk, by Merck Group (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) Rat endothelial cell basal medium, by Cell Applications (1 mentions) Growth supplement, by Cell Applications (1 mentions) Ham s f12, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

3 protocols using rat aortic endothelial cells

1

Heparanase Activity Assay in Rat Aortic Endothelial Cells

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Rat aortic endothelial cells were obtained from Cell Applications. Streptozotocin and other chemicals were obtained from Sigma. Heparin was from Sandoz (10 000 U/mL). Purified HpaL was prepared as described,28 and HpaA was purchased from R&D (#7570‐GH). Yoda1 and 2‐methylthioadenosine 5′‐triphosphate (2‐MeSATP) were purchased from Tocris Bioscience (#5586 and #1062). The G‐actin/F‐actin In Vivo Assay Biochem Kit was obtained from Cytoskeleton Inc. (Denver, CO). ATP Determination Kit was obtained from Invitrogen (#A22066). [3H]‐triolein was purchased from Perkin‐Elmer (NET431001MC). Anti‐heparanase antibody HP3/17 was from Prospec (Rehovot, Israel), which recognizes both the active (50‐kDa) and latent (65‐kDa) form of heparanase. All other antibodies were obtained from Cell Signaling Technology, Santa Cruz Biotechnology, and Millipore, including phospho‐protein kinase D (pPKD; #2054), total protein kinase D (PKD; #SC‐935), phospho‐P38‐mitogen activated protein kinase (pP38MAPK; #9211), total P38‐mitogen activated protein kinase (P38MAPK; #9212), vinculin (#13901), and α‐tubulin (#05–829).
Lee C.S., Zhai Y., Shang R., Wong T., Mattison A.J., Cen H.H., Johnson J.D., Vlodavsky I., Hussein B, & Rodrigues B. (2022). Flow‐Induced Secretion of Endothelial Heparanase Regulates Cardiac Lipoprotein Lipase and Changes Following Diabetes. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 11(23), e027958.
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2

Rat Aortic Endothelial Cell Culture

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Rat aortic endothelial cells were purchased from Cell Applications, Inc. (San Diego, California, United States), and their uptake of DiI-Ac-LDL (CA022K; Toyobo, Osaka, Japan) was confirmed. Cells were routinely cultured in Rat Endothelial Cell Basal Medium with growth supplements (Cell Applications, Inc.). For the experiments, the cells were seeded in 96-well tissue culture plates at a concentration of 20,000 cells per well in Rat Endothelial Cell Basal Medium with growth supplements and grown to confluence.
Iba T., Sasaki T., Ohshima K., Sato K., Nagaoka I, & Thachil J. (2017). The Comparison of the Protective Effects of α- and β-Antithrombin against Vascular Endothelial Cell Damage Induced by Histone in Vitro. TH Open: Companion Journal to Thrombosis and Haemostasis, 1(1), e3-e10.
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3

Isolation and Culture of Rat Vascular Cells

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Rat aortic VSMC and adventitial fibroblasts were isolated and cultured from the abdominal aorta of male Sprague–Dawley rats (Harlan; Indianapolis, IN) using methods described by Gunther et al. and Zhu et al., respectively [38 (link),39 (link)]. Cells were maintained in media containing equal volumes of DMEM (low glucose) and Ham’s F12 (JRH; Lenexa, KS) supplemented with 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA), 100 U/mL penicillin, 100 µg/mL streptomycin and 4 mM L-glutamine, and incubated at 37 °C, 95% air and 5% CO2. Rat aortic endothelial cells were obtained from Cell Applications (San Diego, CA). Cells used for experiments were between passages 4 and 9. Although PROLI/NO was used as an •NO donor in the in vivo experiments due to its efficacy, the short half-life of PROLI/NO makes it impractical to use for cell culture experiments in vitro. Hence, DETA/NO, which has a half-life of 24 h, was used as the •NO donor for all in vitro experiments.
Bahnson E.S., Koo N., Cantu-Medellin N., Tsui A.Y., Havelka G.E., Vercammen J.M., Jiang Q., Kelley E.E, & Kibbe M.R. (2014). Nitric oxide inhibits neointimal hyperplasia following vascular injury via differential, cell-specific modulation of SOD-1 in the arterial wall. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, 44, 8-17.
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