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Apc conjugated anti kit mab

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The APC conjugated anti-Kit mAb is a monoclonal antibody that is conjugated to Allophycocyanin (APC). It is designed for the detection and analysis of cells expressing the Kit (CD117) receptor by flow cytometry.

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Lab products found in correlation

Percp cy5 conjugated lineage specific mabs, by Thermo Fisher Scientific (2 mentions) Phycoerythrin cy7 pe cy7 conjugated anti sca 1 mab, by Thermo Fisher Scientific (2 mentions) Pe conjugated anti flt3 mab, by Thermo Fisher Scientific (2 mentions)

2 protocols using apc conjugated anti kit mab

1

Isolation and Identification of Murine Hematopoietic Stem Cells

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Bone marrow was isolated from SPF and GF mice. The red blood cells were lysed, and 2 × 107 cells were stained with the following monoclonal antibody (mAb) cocktail: peridinin-chlorophyll protein Cy5 (PerCP-Cy5) conjugated lineage specific mAbs (eBioscience; San Diego, CA) (CD11 c, NK1.1, CD11b, Ter119, CD3e, CD8a, CD19, TCRβ, TCRβ, B220, and Gr1), APC conjugated anti-Kit mAb (eBioscience; San Diego, CA), Phycoerythrin/Cy7 (PE/Cy7) conjugated anti-Sca-1 mAb (eBioscience; San Diego, CA), PE conjugated anti-Flt3 mAb (eBioscience; San Diego, CA). Dead cells (propidium iodide–positive) were excluded from the analysis. HSCs were defined as lineage negative, Sca-1 and Kit positive, Flt3 negative cell population.
Spring J., Khan A.A., Lara S., O’Grady K., Wilks J., Gurbuxani S., Erickson S., Fischbach M., Jacobson A., Chervonsky A, & Golovkina T. (2022). Gut commensal bacteria enhance pathogenesis of a tumorigenic murine retrovirus. Cell reports, 40(11), 111341.
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2

Isolation and Identification of Murine Hematopoietic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow was isolated from SPF and GF mice. The red blood cells were lysed, and 2 × 107 cells were stained with the following monoclonal antibody (mAb) cocktail: peridinin-chlorophyll protein Cy5 (PerCP-Cy5) conjugated lineage specific mAbs (eBioscience; San Diego, CA) (CD11 c, NK1.1, CD11b, Ter119, CD3e, CD8a, CD19, TCRβ, TCRβ, B220, and Gr1), APC conjugated anti-Kit mAb (eBioscience; San Diego, CA), Phycoerythrin/Cy7 (PE/Cy7) conjugated anti-Sca-1 mAb (eBioscience; San Diego, CA), PE conjugated anti-Flt3 mAb (eBioscience; San Diego, CA). Dead cells (propidium iodide–positive) were excluded from the analysis. HSCs were defined as lineage negative, Sca-1 and Kit positive, Flt3 negative cell population.
Wang L., Grossman S.R, & Kieff E. (2000). Epstein–Barr virus nuclear protein 2 interacts with p300, CBP, and PCAF histone acetyltransferases in activation of the LMP1 promoter. Proceedings of the National Academy of Sciences of the United States of America, 97(1), 430-435.
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