Chinese hamster ovary cells (CHO-K1) were grown in 75-cm2 cell culture flasks (Corning) in Ham’s F-12 medium (BioWhittaker) supplemented with 10% fetal bovine serum (HyClone) and 1% penicillin-streptomycin (BioWhittaker). Trypsin-Versene (BioWhittaker) was used to detach adherent cells. Polyclonal anti-gp42 antibody serum (PB114) was used as previously described (27 (link)). Monoclonal antibody 3H3 (anti-gp42) was obtained as previously described (11 (link)). The HLA class II DQ monoclonal antibody (1a3) (catalog no. ab24265; Abcam) and anti-FLAG-M2 (catalog no. F1804; Sigma-Aldrich Chemical Company) were used to detect HLA class II and gp42, respectively. Monoclonal anti-FLAG M2 antibody (F1804) and polyclonal anti-FLAG antibody (F7425) were obtained from Sigma-Aldrich Chemical Company.
Polyclonal anti flag antibody f7425
Manufactured by Merck Group
The Polyclonal anti-FLAG antibody (F7425) is a laboratory reagent used for detection and purification of proteins tagged with the FLAG peptide sequence. It is a rabbit-derived polyclonal antibody that specifically binds to the FLAG tag, which is a commonly used protein affinity tag. The antibody can be used in various immunochemical techniques, such as Western blotting, immunoprecipitation, and immunoaffinity chromatography, to identify and isolate FLAG-tagged proteins.
Automatically generated - may contain errors
2 protocols using polyclonal anti flag antibody f7425
1
CHO-K1 Cell Culture and Antibody Detection
2
Epitope-specific Tau and α-Synuclein Antibodies
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Anti-tau antibodies used in this study were as follows: T46 (epitope: 404–441; Thermo Fisher Scientific), pS396 (epitope: p-Ser-396; Calbiochem), RD3 (epitope: 209–224; Millipore), Anti-4R (epitope: 275–291; Cosmo Bio), TauC (epitope: 429–441; Cosmo Bio), AT8 (epitope: p-Ser-202 and p-Thr-205; Thermo Fisher Scientific), tau 360–380 (epitope: 360–380; Cosmo Bio), pS262/pT263 (epitope: p-Ser-262 and p-Thr-263; abcam). Polyclonal anti-α-synuclein (α-syn) 131–140 antibody was obtained from Cosmo Bio. Monoclonal anti-α-tubulin antibody (T9028), monoclonal and polyclonal anti-HA antibodies (H3663 and H6908), and polyclonal anti-FLAG antibody (F7425) were obtained from Sigma.
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