Anti-MTg autoantibodies were determined by ELISA, as described in detail previously (36 (link)). Sera from individual mice were diluted 1/50 or 1/100 and plated in duplicate on MTg-coated ELISA plates. Anti-Ro and anti-La Abs were quantified by ELISA. Briefly, plates were coated overnight with 5 μg/ml anti-Ro Ab (Sigma-Aldrich, St. Louis, MO) or anti-La Ab (Abcam, Cambridge, MA) diluted in PBS. After washing with PBS with 0.5% Tween and blocking with 0.2% BSA in PBS, serum was diluted and incubated on plates overnight. Bound Ig was detected with goat anti-mouse IgG–alkaline phosphatase (0.2 μg/ml; Southern-Biotech, Birmingham, AL) diluted in blocking buffer. Plates were developed with SIGMAFAST p-Nitrophenyl phosphate Tablets (Sigma-Aldrich), and specific absorbance was measured at 405 nm using a Molecular Devices SpectraMax micro-plate reader.
Anti ro ab
Manufactured by Merck Group
The Anti-Ro Ab is a laboratory test used to detect the presence of anti-Ro antibodies in a patient's blood sample. Anti-Ro antibodies are autoantibodies that can be associated with certain autoimmune disorders, such as Sjögren's syndrome and systemic lupus erythematosus. The test is used to aid in the diagnosis and management of these conditions.
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2 protocols using anti ro ab
1
Quantifying Autoantibodies in Mice
2
Quantifying Autoantibodies in Mice
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Anti-MTg autoantibodies were determined by ELISA, as described in detail previously (36 (link)). Sera from individual mice were diluted 1/50 or 1/100 and plated in duplicate on MTg-coated ELISA plates. Anti-Ro and anti-La Abs were quantified by ELISA. Briefly, plates were coated overnight with 5 μg/ml anti-Ro Ab (Sigma-Aldrich, St. Louis, MO) or anti-La Ab (Abcam, Cambridge, MA) diluted in PBS. After washing with PBS with 0.5% Tween and blocking with 0.2% BSA in PBS, serum was diluted and incubated on plates overnight. Bound Ig was detected with goat anti-mouse IgG–alkaline phosphatase (0.2 μg/ml; Southern-Biotech, Birmingham, AL) diluted in blocking buffer. Plates were developed with SIGMAFAST p-Nitrophenyl phosphate Tablets (Sigma-Aldrich), and specific absorbance was measured at 405 nm using a Molecular Devices SpectraMax micro-plate reader.
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