Shodex 804

Manufactured by GE Healthcare

The Shodex 804 is a size exclusion chromatography (SEC) column designed for the analysis of macromolecules such as proteins, polysaccharides, and synthetic polymers. It features a porous resin matrix that separates molecules based on their size and molecular weight. The column is compatible with a variety of aqueous and organic mobile phases, allowing for versatile applications in various fields of study.

Automatically generated - may contain errors

Lab products found in correlation

Astra software package, by Wyatt Technology (2 mentions) Ettan lc, by GE Healthcare (2 mentions) Optilab rex differential refractive index detector, by Wyatt Technology (1 mentions) Optilab rex, by Wyatt Technology (1 mentions)

2 protocols using shodex 804

Hsp27 Oligomerization Analysis

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Solutions of Hsp27 were resolved by analytical SEC on a Shodex 804 column on an Ettan LC (GE Healthcare). Molecular weights were determined by multi-angle laser light scattering using an in-line DAWN HELEOS detector and an Optilab rEX differential refractive index detector (Wyatt Technology Corporation). The column was equilibrated overnight in SEC buffer. Samples were analyzed at the indicated concentrations. Calculation of molecular weights was performed using the ASTRA software package (Wyatt Technology Corporation).

Freilich R., Betegon M., Tse E., Mok S.A., Julien O., Agard D.A., Southworth D.R., Takeuchi K, & Gestwicki J.E. (2018). Competing protein-protein interactions regulate binding of Hsp27 to its client protein tau. Nature Communications, 9, 4563.

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Determination of Hsp27-BAG3 Complex

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Solutions of Hsp27 (15 µM) and BAG3 (either 15 or 30 µM) were pre-mixed and resolved by analytical size exclusion chromatography on a Shodex 804 column on an Ettan LC (GE Healthcare). Molecular weights were determined by multiangle laser light scattering using an in-line DAWN HELEOS detector and an Optilab rEX differential refractive index detector (Wyatt Technology Corporation). The column was equilibrated overnight in BAG buffer prior to analysis. Samples were run at the indicated concentrations. Calculation of molecular weights was performed using the ASTRA software package (Wyatt Technology Corporation).

Rauch J.N., Tse E., Freilich R., Mok S.A., Makley L.N., Southworth D.R, & Gestwicki J.E. (2016). BAG3 is a modular, scaffolding protein that physically links heat shock protein 70 (Hsp70) to the small heat shock proteins. Journal of molecular biology, 429(1), 128-141.

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