Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Invitrogen (Carlsbad, CA, USA). Hemin (ferriprotoporphyrin IX, a potent inducer of HO-1 [13 (link)]) and zinc protoporphyrin IX (ZnPP-IX, a specific HO-1 antagonist [14 (link)]) were the products of Sigma (St. Louis, MO, USA) [15 (link)]. The final concentrations of Hemin and ZnPP-IX were both 0.5 mg/ml. The anti-HO-1 and anti-PPARγ antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). The anti-phospho-NF-κB p65 and anti-α-smooth muscle actin (α-SMA) antibodies were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). GW9662 and rosiglitazone were the products of Cayman Chemical (St. Louis, MO, USA) which were used as PPARγ-specific antagonists and agonists, respectively.
Zinc protoporphyrin 9 znpp 9
Manufactured by Merck Group
Sourced in United States, United Kingdom
Zinc protoporphyrin IX (ZnPP-IX) is a chemical compound used in laboratory equipment. It is a synthetic porphyrin derivative that contains a zinc ion at its center. ZnPP-IX is used as a reference standard and in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Anti phospho nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rosiglitazone, by Cayman Chemical (1 mentions)
Gw9662, by Cayman Chemical (1 mentions)
Anti pparγ, by Cell Signaling Technology (1 mentions)
Anti ho 1, by Cell Signaling Technology (1 mentions)
Anti α smooth muscle actin α sma, by Santa Cruz Biotechnology (1 mentions)
Anti nrf2, by Santa Cruz Biotechnology (1 mentions)
Angiotensin 2 ang 2, by Merck Group (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Anti phospho creb ser133, by Cell Signaling Technology (1 mentions)
Anti pkcε, by Santa Cruz Biotechnology (1 mentions)
Anti creb, by Cell Signaling Technology (1 mentions)
Triciribine, by Merck Group (1 mentions)
3 protocols using zinc protoporphyrin 9 znpp 9
1
Hemin and ZnPP-IX Modulate Cellular Signaling
2
Molecular Signaling Pathways Regulation
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Anti-HO-1 (Cambridge Biosciences Ltd, Cambridge, UK), anti-phospho-PKCδ(Thr505), anti-CREB, anti-phospho-CREB(Ser133) (Cell Signaling, Danvers, MA, USA), anti-phospho-PKCε(Ser729) (Upstate Ltd, Dundee, UK), anti-PKCε, and anti-Nrf2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). TNF-α was from R&D Systems (Abingdon, UK); angiotensin II (Ang II) and zinc protoporphyrin IX (ZnPPIX) from Sigma-Aldrich (Poole, UK).
3
Nrf2-Mediated Cytoprotection in Mice
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Adult male wild-type and Nrf2 À/À mice (C57B/SV129), which were previously described by Xu et al, 27 were bought from Shanghai experiment animal center and used in this study. The study was approved by the institutional animal care and use committee in the Fourth Affiliated Hospital animal center (Harbin Medical University) and carried out in accordance with the Guide for the Care and Use of Laboratory Animals, Eighth Edition (National Academies Press, Washington, DC, 2010). The animals were all housed in the Fourth Affiliated Hospital animal center with a 22 C temperature, 41% relative humidity, and 12-/12-hour light/dark cycles in the animal care wing and were allowed access to water and food ad libitum. Zinc protoporphyrin IX (ZnPP-IX, inhibitor of HO-1), LY294002 (inhibitor of PI3K pathway), and triciribine (inhibitor of Akt pathway) were purchased from Sigma-Aldrich (St. Louis, Missouri). ZnPP-IX (3 mg/kg of body weight), LY294002 (100 mg/kg of body weight), or triciribine (2 mg/kg of body weight) was administered intraperitoneally daily during HBO-PC exposure.
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