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Cay10723

Manufactured by Cayman Chemical
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CAY10723 is a chemical compound. It is a white, crystalline powder. The molecular formula of CAY10723 is C₂₃H₂₈N₂O₂. The molar mass is 364.48 g/mol.

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Lab products found in correlation

Afm30a, by Cayman Chemical (2 mentions) Gsk199, by Cayman Chemical (2 mentions) Foscarnet, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions)

2 protocols using cay10723

1

Inhibition of PAD Enzymes Impacts Viral Infection

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The PAD inhibitors Cl-amidine (Cl-A), BB-Cl-amidine (BB-Cl), GSK199, CAY10727, and AFM30a—also known as CAY10723—were obtained from Cayman Chemical (Ann Arbor). CHX and foscarnet (phosphonoformic acid, PFA) were from Sigma-Aldrich. All these compounds were reconstituted in dimethyl sulfoxide (DMSO) or ethanol accordingly to their solubility. HF4 and Thapsigargin were kindly provided by Dr. P. Thompson and Dr. M. Corazzari, respectively. Immediately before use, the inhibitors were diluted in the cell medium to their final concentrations. For the PADs inhibitors experiments, cells were pre-treated with the inhibitors for 1 h and then infected (MOI 1) by adding the virus without changing the medium. Following virus adsorption (2 h), the viral inoculum was removed, and a new medium with fresh inhibitor was added. After that, no more medium or inhibitor was added until the samples were collected.
Pasquero S., Gugliesi F., Biolatti M., Dell’Oste V., Albano C., Bajetto G., Griffante G., Trifirò L., Brugo B., Raviola S., Lacarbonara D., Yang Q., Sudeshna S., Barasa L., Haniff H., Thompson P.R., Landolfo S, & De Andrea M. (2023). Citrullination profile analysis reveals peptidylarginine deaminase 3 as an HSV-1 target to dampen the activity of candidate antiviral restriction factors. PLOS Pathogens, 19(12), e1011849.
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2

Preparation and Dilution of PAD Inhibitors

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The PAD inhibitors Cl-A, BB-Cl, GSK199, and AFM30a—also known as CAY10723—were purchased from Cayman Chemical (Ann Arbor). All the compounds were solubilized in DMSO according to the manufacturer's instructions. Immediately before use, the inhibitors were diluted in the culture medium to the desired concentrations (Table S1).
Pasquero S., Gugliesi F., Griffante G., Dell’Oste V., Biolatti M., Albano C., Bajetto G., Delbue S., Signorini L., Dolci M., Landolfo S, & De Andrea M. (2022). Novel antiviral activity of PAD inhibitors against human beta-coronaviruses HCoV-OC43 and SARS-CoV-2. Antiviral Research, 200, 105278.
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