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Ldh assay kit

Manufactured by Bioss Antibodies
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The LDH assay kit is a laboratory reagent used to quantitatively measure the activity of the enzyme lactate dehydrogenase (LDH) in biological samples. LDH is an important enzyme involved in cellular metabolism and its levels can provide information about various physiological and pathological conditions.

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Lab products found in correlation

Microplate reader, by Mindray (1 mentions)

2 protocols using ldh assay kit

1

LDH Cytotoxicity Assay Protocol

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The cytotoxicity in damaged cells was assessed by using a lactate dehydrogenase (LDH) assay kit (Bioss, Woburn, MA, USA, AK140). According to the standardized method, we extracted crude enzyme solutions from cells and tissues and sequentially added the reagents. We tested the optical density at 450 OD and calculated the LDH concentration of the samples using the standard curve. The related formulae of LDH activity are as follows: (1) Tissues: LDH (U/g) = 66.67 × LDH concentration (mol/mL)/weight(g); (2) Cells: LDH (U/104 cell) = 0.133 × LDH concentration (mol/mL).
Xia Y., Ding L., Zhang C., Xu Q., Shi M., Gao T., Zhou F.Q, & Deng D.Y. (2023). Inflammatory Factor IL1α Induces Aberrant Astrocyte Proliferation in Spinal Cord Injury Through the Grin2c/Ca2+/CaMK2b Pathway. Neuroscience Bulletin, 40(4), 421-438.
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2

Cytotoxicity and Cardiac Damage Evaluation

Cited 1 time
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The cells were tested using an LDH Assay Kit (AK141; BIOSS) and the cells and mouse cardiac tissue were tested using an CK-MB ELISA Kit (H191-1-1; Nanjing Jiancheng Bioengineering Institute) according to the manufacturers' instructions. Finally, the absorbance was measured at a wavelength of 450 nm using a microplate reader (Mindray Medical International, Ltd.) (27 (link)).
Yuan C., Yang H., Lan W., Yang J, & Tang Y. (2024). Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress. Experimental and Therapeutic Medicine, 27(5), 187.
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