The anti-PD-L1 antibody, anti-KDM1A antibody, and anti-MEF2D antibody were purchased from Abcam. Anti-GAPDH, anti-HA, and anti-Flag were purchased from Sino Biological. Anti-K267me was generated from Abmart by immunization with 263-APSR(meK)PDLR-271 [22 (link)]. ORY-1001 and rotenone were purchased from Sigma. All primers and sgRNAs are shown in Table S1 .
Anti mef2d antibody
Manufactured by Abcam
The Anti-MEF2D antibody is a laboratory reagent used in research applications. It is designed to specifically detect the MEF2D protein, which is a transcription factor involved in various cellular processes. The antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to aid in the study of the MEF2D protein and its role in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh, by Sino Biological (1 mentions)
Anti ha, by Sino Biological (1 mentions)
Anti pd l1 antibody, by Abcam (1 mentions)
Rotenone, by Merck Group (1 mentions)
Anti anp antibody, by Abcam (1 mentions)
Anti β mhc antibody, by Abcam (1 mentions)
Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Ecl plus, by GE Healthcare (1 mentions)
2 protocols using anti mef2d antibody
1
Antibody Validation and Chemical Reagents
2
Western Blot Analysis of Cardiac Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The amount of 40-50 μg protein prepared from mouse myocardium or NMVCs was used in a standard western blot analysis. The polyvinylidene fluoride (PVDF) membrane binding sample protein was incubated with a high affinity anti-ANP antibody (1:500 dilution), anti-ACTA1 antibody (1:500 dilution), anti-β-MHC antibody (1:1000), anti-MEF2D antibody (1:1000)(Abcam, Cambridge, MA). An anti-GAPDH antibody (1:2000) (Santa CruzBiotechnology, USA) was used to detect the level of GAPDH as an internal control. Protein was visualized using the ECL Plus detection system (GE Healthcare, Waukesha, WI).
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