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T chol e test wako

Manufactured by Fujifilm
Sourced in Japan

The T-Chol E-Test Wako is a laboratory equipment product for the quantitative determination of total cholesterol in serum or plasma. It provides an analytical tool for clinical and research applications.

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2 protocols using t chol e test wako

1

Quantification of Serum FGF21, Adiponectin, and Lipids

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Serum FGF21 concentrations were determined using Rat/Mouse (#MF2100) and Human (#DF2100) FGF21 enzyme-linked immunosorbent assay (ELISA) kits (Quantikine ELISA; R&D Systems, Minneapolis, MN, USA). According to the manufacturer’s instruction, recombinant mouse FGF21 had approximately 21% cross-reactivity in the Human ELISA kit, whereas recombinant human FGF21 had 1.4% cross-reactivity in the Rat/Mouse FGF21 ELISA kit. Serum adiponectin concentrations were determined using a Rat/Mouse Adiponectin ELISA kit (#MRP300, Quantikine ELISA; R&D Systems). Serum NEFA, TG, and T-Chol levels were measured using NEFA C-Test Wako (#279-75401), TG E-Test Wako (#432-40210), and T-Chol E-Test Wako (#439-17501) kits (Wako Pure Chemical Industries, Ltd., Osaka, Japan), respectively.
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2

Measurement of Serum Metabolites in Offspring

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Serum NEFA, TG, and T-Chol levels were measured using NEFA C-Test Wako (#279–75,401), TG E-Test Wako (#432–40,210), and T-Chol E-Test Wako (#439–17,501) kits (Wako Pure Chemical Industries, Ltd., Osaka, Japan), respectively19 (link),22 (link). Blood glucose was measured using a glucometer (Glutest PRO R; Sanwa Kagaku Kenkyusho Co., Ltd., Aichi, Japan)22 (link). As milk samples, we used the gastric contents of offspring, which mainly consisted of milk derived from dams, on d1622 (link).
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