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3 protocols using lutrol e400

1

Quercetin-Loaded Nanoemulsion Formulation

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Quercetin was purchased from Himedia, Mumbai. Poloxamer 407, Poloxamer 188 were purchased from Sigma Aldrich, Bangalore, India. Labrafac PG®, Labrasol®, Carbitol®, Transcutol HP® and Capryol 90® were obtained as gift samples from Gatte Fosse, Mumbai, India. Cremophor EL®, Lutrol E400, Carbopol 934P, and Lutrol E600 were obtained as gift samples from BASF Corporation, Mumbai, India. Xanthan gum and Gellan gum were purchased from Triveni Chemicals, Vapi, Gujarat, India. Cinnamon oil and Palm oil were purchased from Genuine Chemical Co., Mumbai. Triacetin, Isopropyl myristate and Castor oil was from Himedia Laboratories Pvt. Ltd., Mumbai, India. Sesame oil was from National Chemicals, Gujarat, India. Migliyol was from Peter Cremer, Hamburg, Germany. Tween 80 and Tween 60 were purchased from Nice chemicals Pvt. Ltd., Kochi and Sisco Research Laboratories Pvt. Ltd., Mumbai, India. Tween 20 and Glycerol were from Merck Specialities Pvt. Ltd., Mumbai, India respectively. Span 80 was from Suvidhinath Laboratories, Baroda. Ethylene glycol was procured from Loba Chemie Pvt. Ltd., Mumbai, India. All other chemicals and reagents were of laboratory grade.
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2

Catalytic Hydrogenation of Cyclic Diol

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Example 2

(Inventive)

3 g of catalyst suspension (Raney copper, 50% in water) were washed three times with methanol. 10 g of 3-methylcyclopentadecane-1,5-diol (II.1) (82.5% by weight by GC) in 20 g of polyethylene glycol (ca. 8 PEG units, Lutrol® E400 from BASF SE, vapor pressure at 180° C.: 0.02 mbar) were then initially charged at room temperature in a 100 ml three-necked flask together with the washed catalyst. The methanol and the residual water were distilled off slowly at 50° C. at a pressure of 250 to 3 mbar. The reaction mixture was then heated to 200° C. at a pressure of 20 mbar. The temperature was maintained for 16 hours. The pressure was then decreased to 1 mbar and the low-boiling components distilled off in one stage. Good mixing was ensured throughout the entire experiment by means of a magnetic stirrer. 4.5 g of distillate with a 14-methyl-16-oxabicyclo[10.3.1]pentadecene content of 87.2 area % by GC could be obtained, which corresponds to a yield of 52%. The 3-methylcyclopentadecane-1,5-diol content was 0.8 area % by GC and the 3-methylcyclopentadecane-1,5-dione content was 3.9 area % by GC.

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3

Antimicrobial Nanocomposite Formulation Development

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Multi-walled carbon nanotubes (MWCNTs), NC7000 series of 90% purity, showing approximately average diameter of 9.5 nm and 1.5 μm length, produced by the catalytic chemical vapor deposition (CCVD) method were purchased from NANOCYL™, Belgium. Gelatin Type B (Bloom strength-240, Pharmaceutical grade, pyrogen free) was purchased from the OPSO Saline Limited, Bangladesh and chitosan of 90% deacetylation degree having molecular mass of 1100 kDa (medicine grade) was purchased from Yuhuan Ocean Biochemistry Co. Ltd, China. Polyethylene glycol (PEG) was purchased from BASF (Lutrol E 400, Macrogol 400 ph. Eur), Bangladesh. All other chemicals and solvents were of reagent grade.
Seven bacterial strains, including 3 gram-positive (Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes) and 4 gram-negative (Escherichia coli 0157, Salmonella enteritidis, Salmonella typhi, Klebsiella pneumoniae) were obtained from the Center for Advanced Research in Sciences (CARS), University of Dhaka, Bangladesh.
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