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Reporter codeset and capture probeset

Manufactured by NanoString
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The Reporter CodeSet and Capture ProbeSet are lab equipment products offered by NanoString. The Reporter CodeSet is a collection of color-coded reporter probes designed to measure the expression levels of specific genes or targets. The Capture ProbeSet is a set of probes used to capture and isolate specific RNA or DNA molecules from a sample. These products enable targeted gene expression analysis and sample preparation for NanoString's nCounter Analysis System, but a detailed description of their core function is limited to avoid potential interpretation or extrapolation.

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Lab products found in correlation

Nsolver 4, by NanoString (2 mentions) Prep station and digital analyzer, by NanoString (2 mentions) Rnaeasy plus, by Qiagen (2 mentions)

2 protocols using reporter codeset and capture probeset

1

NanoString RNA Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Harvested HAECs were washed and lysed, and RNA was isolated using the reagents and procedures supplied in a kit (RNAeasy Plus; Qiagen, Hilden, Germany). RNA quality was confirmed, and concentrations were determined by nanodrop.
The RNA was cleaned and concentrated using a kit (Zymo Research, Irvine, CA) and eluted in RNase-free water. One hundred nanograms was added to the Reporter CodeSet and Capture ProbeSet of the premade Human Inflammation Panel (NanoString Technologies, Seattle, WA) and hybridized overnight at 65°C, per the manufacturer’s protocol. Samples were subsequently processed on a NanoString Technologies Prep station and Digital Analyzer, and the data were analyzed with this company’s nSolver 4.0 Software. Volcano plots were generated by using R version 3.5.0. with packages ggplot2, dplyr, and ggrepel.
Tran-Nguyen T.K., Chandra D., Yuan K., Patibandla P.K., Nguyen K.T., Sethu P., Zhang Y., Xue J., Mobley J.A., Kim Y.I., Shoushtari A., Leader J.K., Bon J., Sciurba F.C, & Duncan S.R. (2020). Glucose-Regulated Protein 78 Autoantibodies Are Associated with Carotid Atherosclerosis in Chronic Obstructive Pulmonary Disease Patients. ImmunoHorizons, 4(2), 108-118.
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2

NanoString RNA Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Harvested HAECs were washed and lysed, and RNA was isolated using the reagents and procedures supplied in a kit (RNAeasy Plus; Qiagen, Hilden, Germany). RNA quality was confirmed, and concentrations were determined by nanodrop.
The RNA was cleaned and concentrated using a kit (Zymo Research, Irvine, CA) and eluted in RNase-free water. One hundred nanograms was added to the Reporter CodeSet and Capture ProbeSet of the premade Human Inflammation Panel (NanoString Technologies, Seattle, WA) and hybridized overnight at 65°C, per the manufacturer’s protocol. Samples were subsequently processed on a NanoString Technologies Prep station and Digital Analyzer, and the data were analyzed with this company’s nSolver 4.0 Software. Volcano plots were generated by using R version 3.5.0. with packages ggplot2, dplyr, and ggrepel.
Tran-Nguyen T.K., Chandra D., Yuan K., Patibandla P.K., Nguyen K.T., Sethu P., Zhang Y., Xue J., Mobley J.A., Kim Y.I., Shoushtari A., Leader J.K., Bon J., Sciurba F.C, & Duncan S.R. (2020). Glucose-Regulated Protein 78 Autoantibodies Are Associated with Carotid Atherosclerosis in Chronic Obstructive Pulmonary Disease Patients. ImmunoHorizons, 4(2), 108-118.
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