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Tetramethylrhodamineisothiocyanate conjugated goat anti rabbit igg

Manufactured by Merck Group
Sourced in United States

Tetramethylrhodamineisothiocyanate-conjugated goat anti-rabbit IgG is a fluorescent-labeled secondary antibody. It is used to detect and visualize target proteins recognized by primary rabbit antibodies in various applications such as immunohistochemistry, Western blotting, and flow cytometry.

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2 protocols using tetramethylrhodamineisothiocyanate conjugated goat anti rabbit igg

1

Lipid Signaling Pathway Analysis

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Anti-nSMase and anti-NOS2 (M-19) were from Santa Cruz Biotechnology Inc. (California, USA). SDS-PAGE molecular weight standard was from Bio-Rad Laboratories (Hercules, CA, USA). VD3 was obtained from DBA Italia (Segrate, Milan, Italy). Dulbecco's modified Eagle's medium (DMEM), bovine serum albumin, tetramethylrhodamineisothiocyanate-conjugated goat anti-rabbit IgG, and MPTP-HCl were from Sigma Chemical Co. (St. Louis, Missouri, USA). Lipid standards 16:0SM, 18:1SM, and 24:0SM were purchased from Avanti (Avanti Polar, Alabaster, AL, USA).
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2

Antibody Sourcing and Utilization

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Most chemicals were purchased from Sigma-Aldrich (St. Louis, MO), except for reagents for 2D-DIGE, which were purchased from GE Healthcare (Uppsala, Sweden). Primary antibodies were purchased from Abcam (Cambridge, UK), except for anti-ARV, which was a kind gift from Dr. L. H. Lee at National Chung Hsing University, Taiwan. Secondary antibodies for immunofluorescence analysis, tetramethylrhodamine isothiocyanate−conjugated goat anti-rabbit IgG and fluorescein isothiocyanate−conjugated goat anti-mouse IgG, were purchased from Sigma-Aldrich. Secondary antibodies for western blotting, horseradish peroxidase−conjugated goat anti-mouse IgG and goat anti-rabbit IgG, were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA).
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