Hemosil d dimer hs 500 kit

All venous blood samples were collected from patients on admission before the pPCI procedure. All the laboratory tests were performed by the emergency laboratory of our hospital. The plasma levels of D-dimer were measured by the immunoturbidimetry method using HemosIL D-dimer HS 500 kit (Werfen, Barcelona, Spain) according to the manufacturer's instruction, and ≤0.5 mg/L was defined as normal. Echocardiography was performed within 24 hours on admission to determine left ventricular ejection fraction (LVEF).

Participants’ fasting, cryopreserved plasma samples were shipped to Temple University (Philadelphia, PA) for centralized quantification of levels of immune/inflammatory biomarkers using enzyme-linked immunosorbent assay kits: soluble CD14 (sCD14; R&D), soluble CD163 (sCD163; IQ Products), MCP-1 (R&D), high-sensitivity IL-6 (R&D), Lp-PLA2 (R&D), and oxLDL (Mercodia) [7 (link)]. D-dimer was measured using the HemosIL D-dimer HS 500 kit on the ACL TOP (Werfen). Fasting, cryopreserved serum samples were shipped to Quest Diagnostics for quantification of high-sensitivity C-reactive protein (hs-CRP) by commercially available assays.

The inclusion criteria of this study were as follows: (i) age > 18 years, (ii) definitive diagnosis of sepsis based on the International Classification of Diseases (ICD 9) code 995.92 (sepsis) and 785.52 (septic shock), which are currently recommended by the Sepsis-3, and (iii) at least one DD value measured within 24 hours after ICU admission (in patients with many sequential DD values available, the first one measured at ICU admission was selected). The plasma DD was assayed using HemosIL D-Dimer HS 500 kit on the Instrumentation Laboratory ACL TOP automated analyzer (Instrumentation Laboratory, Lexington, USA).
The following additional information was then extracted from the database: demographic characteristics, results of laboratory testing and blood gas analysis, severity scores, treatment modalities, comorbidity, length of stay in the ICU, and outcome (i.e., in-hospital death). All laboratory tests, except DD, were collected in the first 24 hours of patient admission. The sequential organ failure score (SOFA) and simplified acute physiology score (SAPSII) scores were calculated as previously described (18 (link), 19 (link)).