The following primary antibodies were used for the experiments: mouse monoclonal antibodies against β-Tubulin, clone TUB 2.1 (Sigma; T4026; 1:500), anti-Nurr1 (Abcam; ab41917; 1:1000) and anti-GFP (Roche; 11814460001; 1:10,000); rabbit polyclonal antibodies against TH (Millipore; AB152; 1:250), anti-Myc (Cell Signaling; 2272; 1:1000), anti-Pitx3 (Abcam; ab30734; 1:1000), anti-pan-synuclein (Abcam; ab6176; 1:500), anti-γ-synuclein (Abcam; ab6169; 1:1000) and anti-CaMKIIβ (Abcam; ab34703; 1:1000). For immunocytochemistry, secondary antibodies conjugated with Cy2/Cy3 fluorophores (Dianova; 115-225-072/111-165-006; 1:500) were used. Nuclear counterstaining was done with 4′, 6′-diamidino-2-phenylindole (DAPI; 2 µg/ml; Invitrogen; D3571). For Western blot, anti-mouse HRP (Dianova; 715-035-150; 1:5000) or anti-rabbit HRP (Dianova; 711-035-152; 1:5000) secondary antibodies were used. Protein levels were normalized to Actin clone c4 (Millipore; MAB1501).
Ab34703
Manufactured by Abcam
Sourced in United States
Ab34703 is a primary antibody product. It is a highly specific antibody designed to detect the target protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab6176, by Abcam (1 mentions)
Mab1501, by Merck Group (1 mentions)
Ab30734, by Abcam (1 mentions)
T4026, by Abcam (1 mentions)
Ab152, by Cell Signaling Technology (1 mentions)
D3571, by Thermo Fisher Scientific (1 mentions)
Ab203491, by Abcam (1 mentions)
Ab128936, by Abcam (1 mentions)
Ab108596, by Abcam (1 mentions)
Ab15191, by Abcam (1 mentions)
Ab9324, by Abcam (1 mentions)
Ab51067, by Abcam (1 mentions)
Sab2100894, by Merck Group (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Ab421, by Abcam (1 mentions)
Ab5577, by Abcam (1 mentions)
Ab222885, by Abcam (1 mentions)
3 3 diaminobenzidine (dab), by Thermo Fisher Scientific (1 mentions)
Ab108400, by Abcam (1 mentions)
Ta 060 qhdx, by Thermo Fisher Scientific (1 mentions)
3 protocols using ab34703
1
Antibody Characterization for Neuronal Markers
2
Comprehensive Protein Extraction and Western Blot Analysis
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Total proteins were extracted from tissues using RIPA Lysis Buffer (Beyotime, Haimen, Jiangsu, China). Equal amounts of the protein were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane, followed by overnight incubation with primary antibodies at 4°C. Primary antibodies against the following proteins were used: Pi3k (1:300, 20584-1-AP, Proteintech), Jak2 (1:4,000, ab108596, Abcam), Gng7 (1:800, GTX65584, GeneTex), Jun (1:1,500, 66313-1-Ig, Proteintech), IL-6 (1:1,000, ab9324, Abcam), Met (1:1,500, ab51067, Abcam), Pdgfra (1:1,000, ab203491, Abcam), Camk2b (1:1,000, ab34703, Abcam), Ptgs2 (1:1,000, ab15191, Abcam), Sdc1 (1:1,000, ab128936, Abcam), and Gapdh (1:10,000; SAB2100894; Sigma–Aldrich; Merck KGaA). Target protein expression was normalized to Gapdh expression.
3
Immunohistochemical Analysis of Lens Proteins
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After deparaffinization and rehydration, the sections were boiled for 10 min in diluted with ddH 2 O from ×100 to ×1 antigen repair solution (MXB Biotechnologies, China) and blocked with 5% BSA for 1 h. Anti-Capn1 monoclonal
antibody (ab108400, ABCAM, USA, 1:100), Anti-Tgm2 polyclonal antibody (ab421, ABCAM, USA, 1:500), Anti-Camk2b polyclonal antibody (ab34703, ABCAM, USA, 1:200), Anti-Gja8 polyclonal antibody (ab222885, ABCAM, USA, 1:100); Anti-Cryab polyclonal antibody (ab5577, ABCAM, USA, 1:200), anti-Cryba1 polyclonal antibody (PA5-71690, ThermoFisher Scientific, USA, 1:500), anti-Crybb1 polyclonal antibody (bs-12582R, Bioss Antibodies, China, 1:500), and anti-Crybb3 polyclonal antibody (21009-1-AP, Proteintech Systems, USA, 1:100) were used as primary antibody. Anti-Rabbit antibody488 (A-21206, Invitrogen, USA), and anti-Rabbit antibody594 (A-21207, Invitrogen, USA) as secondary antibodies were incubated at room temperature for 2 h. Cell nuclei were counterstained with DAPI. DAB (TA-060-QHDX Ther-moFisher Scientific, USA) was used for color development followed by hematoxylin counterstaining in immunohistochemistry assay. Three mice/six lenses from each group were evaluated to compare these proteins expression of these two groups and repeated 3 times.
antibody (ab108400, ABCAM, USA, 1:100), Anti-Tgm2 polyclonal antibody (ab421, ABCAM, USA, 1:500), Anti-Camk2b polyclonal antibody (ab34703, ABCAM, USA, 1:200), Anti-Gja8 polyclonal antibody (ab222885, ABCAM, USA, 1:100); Anti-Cryab polyclonal antibody (ab5577, ABCAM, USA, 1:200), anti-Cryba1 polyclonal antibody (PA5-71690, ThermoFisher Scientific, USA, 1:500), anti-Crybb1 polyclonal antibody (bs-12582R, Bioss Antibodies, China, 1:500), and anti-Crybb3 polyclonal antibody (21009-1-AP, Proteintech Systems, USA, 1:100) were used as primary antibody. Anti-Rabbit antibody488 (A-21206, Invitrogen, USA), and anti-Rabbit antibody594 (A-21207, Invitrogen, USA) as secondary antibodies were incubated at room temperature for 2 h. Cell nuclei were counterstained with DAPI. DAB (TA-060-QHDX Ther-moFisher Scientific, USA) was used for color development followed by hematoxylin counterstaining in immunohistochemistry assay. Three mice/six lenses from each group were evaluated to compare these proteins expression of these two groups and repeated 3 times.
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