NOD.Cg-
PrkdcscidIl2rgtm1WjlTg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ (NSGS) mice and NOD.Cg-
PrkdcscidIl2rgtm1Wj1/SzJ (NSG) mice were purchased from The Jackson Laboratory or bred in-house and maintained under sterile conditions. Established leukemia cell lines or mononuclear cells isolated from samples from patients with AML (1 × 10
6 to 2.5 × 10
6 per mouse) were suspended in PBS and injected into the tail veins of NSG or NSGS mice to establish xenografts. All mice were 4–6 months of age at the time of injection and were male, with the exception of the NOMO-1, MOLM-14:D835Y, and MOLM-14:F691L NSG xenografts, which were established in female mice. Myeloblasts were detected in peripheral blood (patient-derived xenografts) or bone marrow (MOLM-14 xenografts) samples after staining with a
FITC-conjugated anti-human CD45 Ab (BD Biosciences). Samples were analyzed by flow cytometry using a
Gallios flow cytometer and
Kaluza software (both from Beckman Coulter). After engraftment, the mice were weighed and treated once daily with MRX-2843, quizartinib, or vehicle administered by oral gavage in a volume of 10 ml/kg. When mice appeared ill or lost more than 20% of their body weight, they were euthanized.
Minson K.A., Smith C.C., DeRyckere D., Libbrecht C., Lee-Sherick A.B., Huey M.G., Lasater E.A., Kirkpatrick G.D., Stashko M.A., Zhang W., Jordan C.T., Kireev D., Wang X., Frye S.V., Earp H.S., Shah N.P, & Graham D.K. (2016). The MERTK/FLT3 inhibitor MRX-2843 overcomes resistance-conferring FLT3 mutations in acute myeloid leukemia. JCI Insight, 1(3), e85630.
