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Lentivirus

Manufactured by Applied Biological Materials
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Lentivirus is a type of retrovirus that can be used for gene delivery and expression in a wide range of cell types, including non-dividing cells. It serves as a vector for the introduction of genetic material into target cells.

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Lab products found in correlation

Evos fl imaging system, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

QPCR Lentivirus Titration Kit QPCR Lentivirus Titration (Titer) Kit Speedy Lentivirus Purification kit Lentivirus carrying the SALM3 rescue construct Speedy Lentivirus Purification reagent Puro-Blank Lentivirus Lentivirus Titer Kit Ultra-Pure Lentivirus puri cation kits Lentivirus Titration kit Lentivirus qPCR Titer Kit

2 protocols using lentivirus

1

Lentiviral mHTTex1-GFP Expression

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A lentiviral genome encoding mHTT exon 1 with 103 Qs fused to green fluorescent protein (mHTTex1-GFP) (Southwell et al., 2009 ) was packaged into a high titer lentivirus by Applied Biological Materials, Inc (Vancouver, BC).
Caron N.S., Banos R., Aly A.E., Xie Y., Ko S., Potluri N., Anderson C., Black H.F., Anderson L.M., Gordon B., Southwell A.L, & Hayden M.R. (2022). Cerebrospinal fluid mutant huntingtin is a biomarker for huntingtin lowering in the striatum of Huntington disease mice. Neurobiology of disease, 166, 105652.
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2

Transduction of Human OA Chondrocytes

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Extracted and cultured human knee OA chondrocytes were transduced with lentivirus carrying either control-GFP (CTL-GFP) or ZNF440-GFP plasmid (Applied Biological Materials: ABM) as per manufacturer's instructions (vector details are available in Supplemental Table 4). Briefly, resuspended human knee OA chondrocytes (1 Â 10 5 cells/ml) were transduced with lentivirus (MOI 2.5) and cells were transferred into 6 well plates and incubated for 24 h. Culture media was replaced after 3 days with 2 ml complete media containing puromycin and cells were grown to confluence. GFP signals were visualized using the EVOS FL Imaging System (Life Technologies).
Nakamura A., Rampersaud Y.R., Sundararajan K., Nakamura S., Wu B., Matip E., Haroon N., Krawetz R.J., Rossomacha E., Gandhi R., Kotlyar M., Rockel J.S., Jurisica I, & Kapoor M. (2021). Zinc finger protein-440 promotes cartilage degenerative mechanisms in human facet and knee osteoarthritis chondrocytes. Osteoarthritis and cartilage, 29(3).
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