Tracheal aspirate samples were collected within 72 hours of endotracheal intubation with an inline Ballard suction catheter (Halyard, Alpharetta, GA) connected to a sterile Lukens trap (Covidien, Walpole, MA) using up to 5 mL of sterile saline and processed according to published protocols (19 (link), 20 (link)). For infants and toddlers, 1–2 mL of saline is instilled. The Ballard inline suction catheter is passed once to obtain a sample. If 50% of the instilled volume is not returned, then a repeat passage of the Ballard is performed. The Ballard suction catheter (Halyard, Alpharetta, GA) may be flushed with additional saline to move the aspirate into the Lukens trap (Covidien, Walpole, MA). Cell viability was determined with trypan blue exclusion on a Countess hemocytometer. Cell purity was assessed by Shandon Kwik-Diff (Thermo Scientific, Waltham, MA) staining of cytospin cell preparations (20 (link)). Up to 1 × 106 cells were stored in RNALater at –80°C until RNA was extracted for gene expression analysis.
Lukens trap
Manufactured by Medtronic
The Lukens trap is a lab equipment used for the collection and analysis of gas samples. It functions as a device to capture and contain gaseous substances for further examination and testing.
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Lab products found in correlation
2 protocols using lukens trap
1
Tracheal Aspirate Sampling for Gene Expression
2
Tracheal Aspirate Sampling Protocol
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TA samples where obtained within one hour of EBC collection. For the suctioning procedure, bacteriostatic saline (~1 ml) was instilled into the trachea. After several ventilator breaths, an in-line suction catheter (Ballard, Kimberly-Clark, Irving, TX) was inserted just past the endotracheal tube and the sample was then retrieved into a closed, sterile Lukens trap (Covidien, Mansfield, MA). Additional bacteriostatic saline was used if necessary to clear the sample from the suction catheter. Samples were immediately placed on wet ice and transported to the laboratory for analyses.
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