Omega 640 spectrophotometer

Cell proliferation experiments were performed using Cell Titer 96 Aqueous One Solution Cell Proliferation Assay System (Promega, Madison, WI, USA). Briefly, cells were plated in quadruplicate on clear, flat-bottomed 96-well tissue culture treated plates (Corning Costar) and treated with drugs at 37 °C, 5% CO₂. After incubation, MTS reagent was added directly to the wells and incubated for 90 min at 37 °C, 5% CO₂. The absorbance of MTS formazan was immediately read at 490 nm (Omega 640 spectrophotometer, (BMG Labtech, Cary, NC, USA). A blank measurement was taken from the absorbance of the wells with media only and subtracted accordingly. Percentage cell viability was normalized to the absorbance of untreated wells and averaged from the four wells. The data were analyzed with GraphPad Prism 8 software (San Deigo, CA, USA) and represented as percent (%) cell growth relative to control.

Cell proliferation experiments were performed using Cell Titer 96 Aqueous One Solution Cell Proliferation Assay System (Promega, Madison, WI, USA). Briefly, cells were plated in quadruplicate on clear, flat-bottomed 96-well tissue culture plates (Corning Costar) and treated with 0.1 to 50 μM concentrations of XL44. After incubation for 48 h, MTS reagent was added directly to the wells and incubated for 1.5 h. The absorbance of MTS formazan was read at 490 nm (Omega 640 spectrophotometer, BMG Labtech, Cary, NC, USA). A blank measurement was taken for the absorbance of the wells with media only and subtracted accordingly.