Low-molecular-weight chitosan powder and phosphate-buffered saline were purchased from Sigma–Aldrich, Waltham, MA, USA. Antibiotic disks, blotting paper, and bacterial culture media (Mueller–Hinton, Mannitol Salt, Nutrient Agar, Eosin-methylene Blue, and Nutrient Broth Agar) were purchased from Hi-Media Laboratories Pvt. Ltd., Mumbai, India. Vinegar (acetic acid) was purchased from Qualikems Fine Chem Pvt. Ltd., Dhaka, Bangladesh. Chemicals including alcohol and disinfectants were acquired from ZH Chemicals (Hatkhola, Dhaka, Bangladesh). Gram staining materials were obtained from the laboratory stock at the Microbiology and Hygiene Department. All the chemicals were obtained at a purified grade and used as received.
Mannitol salt
Manufactured by HiMedia
Sourced in India
Mannitol Salt is a selective growth medium used in microbiology for the isolation and identification of Staphylococcus species. It contains mannitol, a carbohydrate, and sodium chloride, which inhibits the growth of most other bacteria. Staphylococcus species that can ferment mannitol will produce acid, changing the medium color from red to yellow.
Automatically generated - may contain errors
Lab products found in correlation
Nutrient agar broth, by HiMedia (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Nutrient agar, by HiMedia (1 mentions)
Mueller hinton, by HiMedia (1 mentions)
Eosin methylene blue, by HiMedia (1 mentions)
Low molecular weight chitosan powder, by Merck Group (1 mentions)
Mueller hinton agar (mha), by HiMedia (1 mentions)
2 protocols using mannitol salt
1
Antimicrobial Susceptibility Testing Protocol
2
Hesperidin-Loaded PLGA Nanoparticles
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Sodium acetate, sodium carbonate, n-butanol, sodium hydroxide, acetone, ethanol, chloroform, aluminum chloride 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), α, α-diphenyl-β-picrylhydrazyl (DPPH) and were purchased from Merck, Mumbai, India. Mannitol salt, Mueller Hinton Agar were obtained from Hi-media Ltd. All the chemicals used were of analytical grade. Hesperidin-loaded PLGA nanoparticles (POL-PLGA-NPs) were oil in water single emulsion solvent evaporation method, and its physiochemical features were confirmed by Transmission Electron Microscopy (TEM), dynamic light scattering (DLS), Fourier Transform Infrared Spectroscopy (FTIR), XRD and NMR analysis [23] (link).
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